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This paper reports the completion of fundamental fluidic operation considered essential to build digital microfluidics system, which can be used for micro total analysis system (mu-TAS) or lab-on-a-chip by the coplanar electrode type of EWOD device. Coplanar electrode type of EWOD chip could be designed as a novel tool to delivery many kinds of bio-samples, such as DNA, enzyme, and salt solution without...
A novel optical biosensor with gold surface bound silver nanoparticles has been constructed for detection of bio-molecules like antigen, antibody protein and DNA plasmid, which exhibited distinct optical properties of localized surface plasmon resonance (LSPR). The silver colloidal nanoparticles were prepared by using sodium borohydride reduction of silver nitrate and characterized by using UV-vis...
We demonstrate that the efficiency of DNA hybridization could be improved by introducing elevated temperature in the hot region and higher velocities in the cold region of a microfluidic chip. Compared with the conventional methods, this hybridization microchip was shown to increase the hybridization signal 4.6-fold within 30 minutes using a 1.4 kb target DNA as the test material. The increase in...
Microarray is one of the most powerful DNA detection systems with multiplexing and high throughput capability. However, the current application of microarray is limited to the preliminary genomic screening of biological samples despite of its excellent potentials for other applications, such as biosensing. This paper addresses microarray's possibilities as sensing device by investigating its limit...
Diffusion is often the rate-limiting step in microfluidic-based biochemical analysis systems. In this paper, an alternative current electrothermal mixer for molecular diagnostic is presented. The mixer consists of a concentric electrode design integrated in a microfluidic cartridge. We investigated the electrothermal flow by visualizing the trajectories of seeded particles. The velocity shows a fourth...
An unique, sensitive, and highly specific immunoassay system using gold nanoparticles and surface-enhanced Raman spectroscopy (SERS) is described. To demonstrate the analytical capabilities of the new technique, Raman reporter (DTNB) that is coimmobilized with biospecific anti-protein A on gold nanoparticles (AuNP). Anti-protein A-AuNP-DTNB, which combing both electromagnetic field and chemical enhancement,...
Enzyme free glucose micro-biosensors with nanoporous platinum (Pt) electrode were fully packaged with biocompatible Nafion membrane and characterized in bio-fluidic solutions. Three different packaging methods which were a dip coating, spin-casting, and chemical bonding were applied, characterized, and compared to find an optimal one for anti-fouling of protein in bio-fluidic solution. In human plasma...
This paper reports a rapid, simple and direct method for the synthesis of gold nanoparticles (AuNPs) in polypyrrole (PPy) film, which provides a biocompatible matrix for the strong adsorption of antibodies. The electrochemical synthesis of AuNPs/PPy composite on gold or carbon electrode occurs within 20 minutes, and does not involve addition of any nanoparticles or reducing agent. This method is compatible...
Use of this work proposes a novel technique to induce the attachment of tumor cells to the micropatterns of glutaraldehyde (GA)-crosslinked gelatin. It provides another method to crosslink gelatin other than using the photo-sensitizing agents or selective GA-crosslinked technique. The micropatterns of GA-crosslinked gelatin are fabricated by O2 plasma etching process. This novel technique can ensure...
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