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The paper presents an integrated automatic micro reverse transcription polymerase chain reaction (RT-PCR) system for molecular diagnosis. The developed system can detect viruses with a high sensitivity and specificity using antibody-conjugated superparamagnetic beads. The target viruses were first captured by conjugated antibodies on the magnetic beads and then attracted by a magnetic field generated...
Fraction collection following DNA electrophoresis has many applications in biology analysis. These assays typically need to identify specific DNA fragments in the separated sample for further processing and require extraction of one or a group of fragments. Unfortunately, the conventional methods involve cumbersome procedure and are not amenable to integration, automation and extraction of microscale...
The reduction in dimensions of biochip arrays is of interest only if the size of the spots containing DNA or proteins is kept uniform. This condition is necessary to properly compare fluorescence signals from the different spots and thus to validate the results. Toward this aim, we propose a fully automated MEMS-based spotter enabling the spatial positioning of the depositing cantilever array and...
5SPE (Solid phase extraction) method, which is compatible with MEMS (micro-electro-mechanical system) technology, has proven efficient and amenable to microfluidic chips. An SPE microfluidic chip for DNA extraction by using porous silicon dioxide as the solid phase matrix has been developed. Porous matrix coated on the wall of the channel in the microfluidic chip to provide a surface-enlarging matrix,...
The current real-time PCR (polymerase chain reaction) platforms, which can detect and quantify several target DNA simultaneously, are equipped with discrete optics and detectors for different fluorescence wavelengths. However, the optical loss, due to the different lengths of the channels for several dyes, lowers the performance of fluorescence detection. Especially on the PCR platforms of lab-on-a-chip...
We describe a prototype micro-laboratory for rapid genetic identification of bacterial pathogens from infected human body fluids. The core module of the detection platform is a microfabricated electrochemical sensor array. Picomolar amperometric detection is achieved through formation of a DNA sandwich between capture and detector probe pairs and bacterial 16S rRNA, coupled with an oxidoreductase...
This paper presents a CMOS biochip for DNA detection with an embedded current amplifier circuit by using self-assembly multilayer gold nanoparticles (AuNPs). The biochip is fabricated by a TSMC 0.35 mum 2P4M standard CMOS process and post-CMOS micromachining processes. On each measuring spot, there is a pair of electrodes with a gap distance of 350 nm. Through a self-assembly process between suspended...
Gold-coated microcantilevers were used to develop sensitive micromechanical biosensor for detecting mismatch mutations in DNA hybrids. The selective 5'-end immobilization of 25-mer single-stranded (ss) DNA probes to a gold-coated cantilever surface and the specific hybridization of ssDNA targets to the immobilized probe were performed by the direct electric field control. The controlled electric fields...
Different from traditional detection methods including polymerase chain reaction (PCR) based assays and enzyme-linked immunosorbent assays (ELISA) which are expensive and time consuming for biochemical analysis, in our study, a DNA chip assay using gold nanoparticle (AuNP) immunological amplification method and RFID communication platform were applied to detect DNA sequences rapidly and conveniently...
A new type of bio-chemical sensor based on SPM was designed and fabricated in this paper. The bio-chemical sensor uses the SPM head as the probe part, which can convert the physical variation quantity on the micro-cantilever to electrical signals, and finally change it into image. In the experiment, the bio-chemical sensor based on SPM is used to detect two kinds of ssDNA molecules in PBS solution...
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