The zona pellucida (ZP) is an important component of the oocyte. The stiffness of ZP plays crucial roles in many applications, such as nuclear transplantations (NT), embryo micro-injections, and clone. While conventional methods usually evaluate the deformability of the oocytes assuming the oocytes in static equilibrium when deforming, a novel evaluation obtained from the dynamic deformation is utilized in this work. In this paper, a method based on the subpixel cell contour detection algorithm for evaluating the zona pellucida of the porcine oocytes' deformability is proposed. A subpixel cell contour detection algorithm is used to detect the edge of the porcine oocytes to get the deformation dynamic curve; a self-developed pneumatic micro-injection system is used to apply pressure on the oocyte to deform the oocyte, and a transfer junction is got to evaluate the deformability of the porcine oocytes. The R2 of the fitted model is 0.9477.