This paper describes a batch release technique for monodisperse liposomes immobilized on a substrate (Fig. 1). A single short-time pulsed voltage to the substrate induced detachment of the arrayed liposomes previously developed. Simultaneous release was observed shortly after the electrical stimulation. The release technique produced monodisperse and solvent-free liposomes freely suspended on the substrate, and allowed manipulation of the liposomes. The technique extends the application of our liposome array platform regarding collection/selection of the liposomes interested.