This study is based on the tests of the fitness and health of Year 2007 college girls in Jinggangshan University, located in the center of Jiangxi Province. The tests were performed in 2007 and 2010 twice. The result shows that the BMI and strength (grip strength) for the girl students has improved significantly (P<0.01), while the athletic performance (standing long jump) and the cardiopulmonary function has worsen (P<0.05) after four-year college life in 2010. From the test results, the overall quality of college girls' fitness has the downward trend and this should be paid close attention.