In this study, a system for detection and tracking of eye movements has been developed. The system includes a video camera capable of making Infrared (IR) imaging, near-IR illumination system and record/analysis software. System tracks eye movements by detecting the location of pupil based on features of anatomic structure of the eye and the reflection on the cornea of the light source as the first corneal reflection. Experimental results show that system might successfully be used in different applications related to human computer interaction.