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High-Throughput Endpoint and Real-Time Detection of Biochemical Reactions in Microarrays Using Label-Free Oblique-Incidence Reflectivity Difference Microscopes
We describe recently developed oblique-incidence reflectivity difference microscopes, a form of polarization-modulated imaging ellipsometry, for label-free detection of biochemical reactions in microarrays. Configurations enabling high-throughput endpoint and real-time detection on glass substrates are discussed.