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Monoclonal antibodies (mAbs) are major reagents for research and clinical diagnosis. For their inherently high specificities to intended antigen targets and thus low toxicity in general, they are pursued as one of the major classes of new drugs. Yet binding properties of most monoclonal antibodies are not well characterized in terms of affinity constants and how they vary with presentations and/or...
Using a combination of an oblique-incidence reflectivity difference (OI-RD) scanning microscope and a customized 8-chamber sample cartridge, we detect 300 surface-immobilized molecular targets reacting with up to 8 different analytes simultaneously on a single slide.
We investigated surface chemistry platforms for immobilizing two types of synthetic, small-molecule compound libraries, each with over 6000 compounds, and for screening these compounds for potential proteins ligands by a label-free optical scanning microscope.
We study the kinetic effect of extrinsic fluorescent labeling agents on protein-ligand binding affinity and find that the kinetics is related to the loss or change of protein function when proteins are fluorescent-labeled.
We describe a novel scanning optical microscope that enables high-throughput label-free detection of end-points and kinetics of multiple biomolecular reactions on microarrays with more than 10,000 protein or small-molecule targets.
The potential of biomolecular microarrays on glass for high-throughput kinetics assays has not previously been fully exploited. We demonstrate real-time label-free optical detection of antibodies binding to drug-antigen microarrays using oblique-incidence reflectivity difference (OI-RD) microscopes.
We describe a novel scanning optical microscope that enables high-throughput label-free detection of end-points and kinetics of multiple biomolecular reactions on microarrays with more than 10,000 protein or small-molecule targets.
We study the kinetic effect of extrinsic fluorescent labeling agents on protein-ligand binding affinity and find that the kinetics is related to the loss or change of protein function when proteins are fluorescent-labeled.
We describe recently developed oblique-incidence reflectivity difference microscopes, a form of polarization-modulated imaging ellipsometry, for label-free detection of biochemical reactions in microarrays. Configurations enabling high-throughput endpoint and real-time detection on glass substrates are discussed.
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