Hairy root cultures of Solanum trilobatum L. were established from leaf explants infected with Agrobacterium rhizogenes strains viz., MTCC 532, MTCC 2364 and ATCC A 4 . Initially 27 independent root clones were obtained and 5 clones (ST-02, ST-09, ST-11, ST-13 and ST-17) were scrutinized based on their rapid growth in MS solid medium and exemplified for biomass and solasodine accumulation. MS liquid medium containing 3% sucrose with an initial pH of 5.8 favored the maximum biomass accumulation (27.5 fold increments than initial inoculums) of hairy roots. Amid 5 clones, ST-09 was found to the superior one, producing 2 fold greater biomass (3.85±0.11g FW) and 3.3 fold elevated solasodine content (4.76±0.05mg/g DW) than non transformed roots (control). PCR and southern hybridization analysis revealed the integration of rolA gene in transformed roots. Hairy roots of ST-09 elicited with 4μM methyl jasmonate (MJ) for 2 weeks resulted in 1.9 fold and 6.5 fold enhanced production of solasodine (9.33±0.04mg/g DW) than unelicited and non transformed roots, respectively. This was also corroborated by RT-PCR analysis, showing a considerable upregulation of hmgr (HMGCoA reductase) gene transcripts during 1st 2 weeks of elicitation. A significant increase was noticed in the total phenolics (150.42mg/g DE), total flavonoids (521.09mg/g DE) and radical scavenging activity (83.3%) of 4μM MJ elicited roots in comparison with control. Thus, the study illustrates that hairy roots of S. trilobatum could be exploited for scaling up in bioreactors for the large-scale production of solasodine.