We report a simple approach to reduce graphene oxide (GO) solution by pulsed laser irradiation. The reduction was rapidly carried out at room temperature in only 5min. The reduced graphene oxide (r-GO) was characterized with UV–visible spectroscopy, X-ray diffraction, Raman spectroscopy, X-ray photoelectron spectroscopy, thermo-gravimetric analysis, Fourier transform infrared spectroscopy, scanning electron microscopy and atomic force microscopy. Based on this reducing method, an r-GO conductive film with a sheet resistance of 53.8kΩ/sq was obtained. The pulsed laser reduction of GO in solution creates a new way to produce graphene composites for a variety of applications.