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Department of Biological Sciences, Kyoto University Faculty of Medicine, Kyoto University, Sakyo, Kyoto 606-8507, Japan Department of Medical Embryology and Neurobiology, Institute for Frontier Medical Sciences, Kyoto University, Shogo-in, Sakyo, Kyoto 606-8507, JapanWe report a novel zygotic gene encoding a Xenopus endodermal nuclear factor, Xenf. Expression of Xenf starts at the late blastula stages and is decreased after gastrulation. Xenf shows no structural homology to any known proteins. When GFP-tagged Xenf is overexpressed in Xenopus cells, Xenf protein is localized to the nucleus, associating closely with the chromosomes. In animal cap assays, Xenf expression is strongly activated by mRNA injection of Vg1 and VegT, maternal vegetal genes that can induce endodermal differentiation. In contrast, Xenf is not induced by endoderm-inducing zygotic transcription factors such as Sox17 and Mix-related genes. In turn, Xenf does not activate expression of Sox17, Mixer or Milk. Thus, Xenf is regulated by maternal vegetal positional information in a parallel manner to Sox17 and Mix-related gene pathways.XenfEndodermXenopusEmbryogenesisTranscription factorSox17MixerMilkVg1VegT
Department of Biological Sciences, Kyoto University Faculty of Medicine, Kyoto University, Sakyo, Kyoto 606-8507, Japan Department of Medical Embryology and Neurobiology, Institute for Frontier Medical Sciences, Kyoto University, Shogo-in, Sakyo, Kyoto 606-8507, JapanWe report a novel zygotic gene encoding a Xenopus endodermal nuclear factor, Xenf. Expression of Xenf starts at the late blastula stages and is decreased after gastrulation. Xenf shows no structural homology to any known proteins. When GFP-tagged Xenf is overexpressed in Xenopus cells, Xenf protein is localized to the nucleus, associating closely with the chromosomes. In animal cap assays, Xenf expression is strongly activated by mRNA injection of Vg1 and VegT, maternal vegetal genes that can induce endodermal differentiation. In contrast, Xenf is not induced by endoderm-inducing zygotic transcription factors such as Sox17 and Mix-related genes. In turn, Xenf does not activate expression of Sox17, Mixer or Milk. Thus, Xenf is regulated by maternal vegetal positional information in a parallel manner to Sox17 and Mix-related gene pathways.XenfEndodermXenopusEmbryogenesisTranscription factorSox17MixerMilkVg1VegT
Department of Biological Sciences, Kyoto University Faculty of Medicine, Kyoto University, Sakyo, Kyoto 606-8507, Japan Department of Medical Embryology and Neurobiology, Institute for Frontier Medical Sciences, Kyoto University, Shogo-in, Sakyo, Kyoto 606-8507, JapanWe report a novel zygotic gene encoding a Xenopus endodermal nuclear factor, Xenf. Expression of Xenf starts at the late blastula stages and is decreased after gastrulation. Xenf shows no structural homology to any known proteins. When GFP-tagged Xenf is overexpressed in Xenopus cells, Xenf protein is localized to the nucleus, associating closely with the chromosomes. In animal cap assays, Xenf expression is strongly activated by mRNA injection of Vg1 and VegT, maternal vegetal genes that can induce endodermal differentiation. In contrast, Xenf is not induced by endoderm-inducing zygotic transcription factors such as Sox17 and Mix-related genes. In turn, Xenf does not activate expression of Sox17, Mixer or Milk. Thus, Xenf is regulated by maternal vegetal positional information in a parallel manner to Sox17 and Mix-related gene pathways.XenfEndodermXenopusEmbryogenesisTranscription factorSox17MixerMilkVg1VegT
Department of Biological Sciences, Kyoto University Faculty of Medicine, Kyoto University, Sakyo, Kyoto 606-8507, Japan Department of Medical Embryology and Neurobiology, Institute for Frontier Medical Sciences, Kyoto University, Shogo-in, Sakyo, Kyoto 606-8507, JapanWe report a novel zygotic gene encoding a Xenopus endodermal nuclear factor, Xenf. Expression of Xenf starts at the late blastula stages and is decreased after gastrulation. Xenf shows no structural homology to any known proteins. When GFP-tagged Xenf is overexpressed in Xenopus cells, Xenf protein is localized to the nucleus, associating closely with the chromosomes. In animal cap assays, Xenf expression is strongly activated by mRNA injection of Vg1 and VegT, maternal vegetal genes that can induce endodermal differentiation. In contrast, Xenf is not induced by endoderm-inducing zygotic transcription factors such as Sox17 and Mix-related genes. In turn, Xenf does not activate expression of Sox17, Mixer or Milk. Thus, Xenf is regulated by maternal vegetal positional information in a parallel manner to Sox17 and Mix-related gene pathways.XenfEndodermXenopusEmbryogenesisTranscription factorSox17MixerMilkVg1VegT
Department of Biological Sciences, Kyoto University Faculty of Medicine, Kyoto University, Sakyo, Kyoto 606-8507, Japan Department of Medical Embryology and Neurobiology, Institute for Frontier Medical Sciences, Kyoto University, Shogo-in, Sakyo, Kyoto 606-8507, JapanWe report a novel zygotic gene encoding a Xenopus endodermal nuclear factor, Xenf. Expression of Xenf starts at the late blastula stages and is decreased after gastrulation. Xenf shows no structural homology to any known proteins. When GFP-tagged Xenf is overexpressed in Xenopus cells, Xenf protein is localized to the nucleus, associating closely with the chromosomes. In animal cap assays, Xenf expression is strongly activated by mRNA injection of Vg1 and VegT, maternal vegetal genes that can induce endodermal differentiation. In contrast, Xenf is not induced by endoderm-inducing zygotic transcription factors such as Sox17 and Mix-related genes. In turn, Xenf does not activate expression of Sox17, Mixer or Milk. Thus, Xenf is regulated by maternal vegetal positional information in a parallel manner to Sox17 and Mix-related gene pathways.XenfEndodermXenopusEmbryogenesisTranscription factorSox17MixerMilkVg1VegT