Compare the muscle activation patterns of the quadricep-hamstring during drop jumps with increasing demands of drop heights.Observational.University biomechanics laboratory.Fifteen male and eight female college physical education students.Electromyographic activity of the rectus femoris (RF) and biceps femoris (BF) during the landing and takeoff phase of drop jumps from 20 to 60-cm heights. The ground contact time, vertical ground reaction force (vGRF), knee flexion angle during ground contact, and jump height after takeoff were also analyzed.The activation of RF was higher in the drop jump from 60-cm than that from 20- and 30-cm (comparing 107.0±45.9 to 82.3±30.8 and 88.9±38.9 %MVIC, P<.05) during the landing phase. Activation of BF remained similar across all drop heights. Drop jump from 60-cm resulted in greater contact time during takeoff phase and peak vGRF, and resulted in greater maximum knee flexion but straighter knee at ground contact than from lower drop heights.At drop height of 60-cm, the altered knee muscular activation and movement patterns may diminish the effectiveness of plyometric training and increase the potential injury risk of knee.