Graphene oxides (GO) and reduced graphene oxides (RGO) have gained increasing attention for high-capacity lithium ion batteries (LIBs). However, the serious capacity fading of graphene and RGO due to layer re-stacking has limited their application. In this study, we found that by infiltrating homogeneously pristine GO with O/C ratios up to 0.3 in conductive matrix, the GO anode exhibits much better cyclic performances as compared with RGO. In-operando Raman spectroscopy and density functional theory (DFT) simulations indicate that, owing to the spatial effect of the rich oxygen groups, GO shows increased inter-layer stability for Li storage. The (de)lithation process of GO is highly reversible, and the framework of GO varies little during the charge/discharge circles.