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Fermentation process for tetrameric human collagen prolyl 4-hydroxylase in Escherichia coli: Improvement by gene optimisation of the PDI/β subunit and repeated addition of the inducer anhydrotetracycline
Collagen Research Unit, Biocenter Oulu and Department of Medical Biochemistry and Molecular Biology, P.O. Box 5000, University of Oulu, FIN-90014 Oulu, Finland
Bioprocess Engineering Laboratory, Biocenter Oulu and Department of Process and Environmental Engineering, P.O. Box 4300, University of Oulu, FI-90014 Oulu, Finland
Bioprocess Engineering Laboratory, Biocenter Oulu and Department of Process and Environmental Engineering, P.O. Box 4300, University of Oulu, FI-90014 Oulu, Finland
Bioprocess Engineering Laboratory, Biocenter Oulu and Department of Process and Environmental Engineering, P.O. Box 4300, University of Oulu, FI-90014 Oulu, Finland
Bioprocess Engineering Laboratory, Biocenter Oulu and Department of Process and Environmental Engineering, P.O. Box 4300, University of Oulu, FI-90014 Oulu, Finland
Collagen Research Unit, Biocenter Oulu and Department of Medical Biochemistry and Molecular Biology, P.O. Box 5000, University of Oulu, FIN-90014 Oulu, Finland
Bioprocess Engineering Laboratory, Biocenter Oulu and Department of Process and Environmental Engineering, P.O. Box 4300, University of Oulu, FI-90014 Oulu, Finland