Objective: Transportation of oocytes instead of patient transfer from remote clinic to assisted reproductive technology (ART) laboratory in intracytoplasmic sperm injection (ICSI) program not only helps patients physically but also helps remote clinic financially by saving the cost to set up facilities for ICSI. The influence of oocyte transportation on fertilization and early embryo development in human ICSI program has been studied.Design: Fertility and developmental competence of the oocytes non-transported (control) and transported from two remote clinics, 30 minutes (TP-30) and 90 minutes (TP-90) distance were examined.Materials and Methods: The patients in ICSI program received gonadotrophin releasing hormone agonist (GnRHa) + human menopausal gonadotrophin (HMG) regimens. A dose of 10000 IU human chorionic gonadotrophin (HCG) was administered when two dominant follicles reached 18 mm diameter. Thirty six hours after HCG, the oocytes were recovered transvaginally using ultrasound guidance. Oocytes were washed 4-5 times in mHTF-Hepes, placed in a cryovial containing 1.0 ml of the medium and 0.8 ml of prewashed oil, and transported in a 37°C thermos to the ART laboratory. Three hours after aspiration, cumulus cells surrounding the oocytes were removed by 0.1% hyaluronidase treatment and repeated pipetting. Liquified semen from oligozoospermic patients was washed with mHTF-Hepes, and a single living immobilized spermatozoa was injected into an oocyte on a warm stage. The sperm injected oocytes were cultured in 30 ul drop of mHTF supplemented with 10% synthetic serum substitute (SSS). Eighteen hours after microinjection, oocytes were inspected for normal fertilization by assessing the presence of two pronuclei. Fertilized oocytes were cultured in 30 ul drop of mHTF supplemented with 10% heat-inactivated follicular fluid for 24 hours and the cleaved embryos were observed. The data were analysed by Analysis of Variance.Results: ICSI was performed on 270 oocytes from 42 patients (147 control, 52 TP-30, 80 TP-90). The rates of fertilization and lysis were 75.5% and 12.2%, 69.2% and 15.4%, 75.0% and 10.0% for control, TP-30 and TP-90, respectively. The cleavage rates and the percentage of grade 1 embryo among cleaved embryos were 75.5% and 70.3%, 69.2% and 77.8%, 75.0% and 83.3% for control, TP-30, and TP-90, respectively. There were no differences in the fertilization, cleavage, and percentage of grade 1 embryos (p > 0.05).Conclusion: Transportation of oocytes within 90 minutes after oocyte collection does not affect ICSI results. However, the development of transported oocytes to blastocyst stage remains to be studied.