An amplified colorimetric detection of Hg 2+ is proposed by combining T–Hg 2+ –T base pairs and hybridization chain reaction (HCR). Two hairpins consisting of three-fourths and one-fourth of the horseradish peroxidase (HRP)-mimicking DNAzyme in inactive configuration are used as functional elements. In the presence of Hg 2+ , one of the hairpins is opened by an assistant probe with the help of T–Hg 2+ –T base pairs and this triggers an autonomous cross-opening of the two hairpins using the strand displacement principle, resulting in the formation of DNA nanowires consisting of numerous reunited Q-quadruplex DNAzyme units. The resulting catalytically active hemin/G-quadruplex HRP-mimicking DNAzymes catalyze the oxidation of ABTS 2− by H 2 O 2 into a green-colored cationic radical ABTS •+ for the colorimetric readout. This “turn-on” sensing system enables the high sensitive and selective detection of aqueous Hg 2+ with a detection limit of 9.7pM.