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We previously reported the development of genomic‐DNA‐based high‐resolution genotyping methods for SLA‐DQB1 and DRB1. Here, we report the successful typing of SLA‐DQA using similar methodological principles. We designed a method for comprehensive genotyping of SLA‐DQA using intronic sequence information of SLA‐DQA exon 2 that we had obtained from 12 animals with different SLA‐DQB1 genotypes. We expanded...
In an attempt to enable comprehensive high‐resolution genotyping of the swine leukocyte antigen (SLA) gene, we performed a systemic analysis of nucleotide polymorphisms at introns 1 and 2 and exon 2 from diverse alleles of SLA‐DRB1 and DRB1 pseudogenes. We amplified and cloned 16 partial sequences of SLA‐DRB1 and DRB2 introns 1 and 2 from different alleles, and analyzed them together with sequences...
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