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An important objective in stem cell research is controlling differentiation of pluripotent stem cells to a desired fate. Previous research in this area has focused on directing differentiation by manipulating morphogens and substrate material/mechanics. However, cell-cell signaling, whether by contact or paracrine signaling, also influences differentiation. One promising direction by which cell-cell...
Laser direct-write (LDW) is a biofabrication method capable of rapidly creating precise patterns of viable cells. While previous studies have successfully printed cells to polystyrene Petri dishes, here we have adapted LDW to printing both human dermal fibroblasts and mouse embryonic stem cells (mESCs) to cover slips, allowing more efficient use of culture reagents. Viability of both cell types was...
Unified electro-optical microprobes for co-localized optical and electrical recordings within a rats brain have been recently introduced (Krapohl, Bonin et al. 2008). In this work, we show a simplified production method for these multimodal multisite neuroprobes. The electro-optical neuroprobe has an outer diameter of 255 mum and is composed of eight NiChrome wires (Oslash 25 mum) fixed to the surface...
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