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Microsecond pulsed electric fields (μsPEFs) with amplitude of tens of kV/m are used to permeabilize the plasma membrane whereas nanosecond pulsed electric fields of MV/m also permeabilize cell internal structures, such as the endoplasmic reticulum. In this work, a numerical realistic model of cell and its reticulum has been realized to study the use of μsPEFs also for the permeabilization of this...
Background Human mesenchymal stem cells are promising tools for regenerative medicine due to their ability to differentiate into many cellular types such as osteocytes, chondrocytes and adipocytes amongst many other cell types. These cells present spontaneous calcium oscillations implicating calcium channels and pumps of the plasma membrane and the endoplasmic reticulum. These oscillations regulate...
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