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This paper reports a novel click-crosslinkable and photodegradable gelatin hydrogels, which is useful for micropatterned three-dimensional culture. The azide-modified gelatin was prepared by mixing gelatin with Azide-PEG4-NHS ester solutions. The DBCO-PC-4armPEG was synthesized by the reaction between NHS-PC-4armPEG and DBCO-PEG4-amine. PD-gelatin was performed by mixing solutions of azide-modified...
In this work, we present a novel photocleavable crosslinker to form photodegradable hydrogel through one step reaction by mixing with biocompatible polymers containing amino moieties (amino-terminated tetra-arm poly(ethylene glycol) or gelatin). Our new photocleavable crosslinker can be utilized as potentially versatile and convenient material to prepare photodegradable hydrogels with amino terminated...
The goal of this article is to make a pattern of human induced pluripotent stem cells (hiPSCs) on a Polydimethylsiloxane (PDMS) surface. Here, we report that pattering of hiPSCs can be achieved by patterned plasma treatment followed by coating with a two protein mixture. The result is useful for future applications of micro devices in human cell culture.
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