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In this work, FLIM-FRET experiments are performed in HeLa cells expressing a genetically encoded Ca2+ indicator (GECIs) based on FRET, called Cameleon probes. These sensors allow the analysis of Ca2+ dynamics in different cell compartments, such as mitochondria and cytosol. Cameleons are composed by two fluorescent proteins (FPs, donor and acceptor moieties) and two Ca2+ responsive elements that alter...
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