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In recent years, natural polymer‐based electrospun fibers (EFs) with huge specific surface area, good biocompatibility, and biological activity obtained from electrospinning process exhibit tremendous vitality in the field of biomedical areas. Herein, the parameters of electrospinning from two perspectives, polymer solution such as solvent, polymeric relative molecular mass, concentration, viscosity,...
Immunoassay is one of the most important protein detection methods, which is widely used in clinical diagnosis, environment monitor and biological research due to its high specificity and sensitivity. Most of the existing protein immunoassay detection devices have the weakness of high expenses, large scale and low sample processing rate. Microfluidic immunoassay chips have been widely recognized as...
Objective To investigate the culture method of melanocytes in melasma skin, and observe the morphology and biological properties of the cultured melanocytes in vitro. Methods The skin specimen was obtained from melasma patients after lower blepharoplasty. Epidermis was isolated by 0.25% dispase, and then single cell suspension was obtained after digested by 0.25% trypsin and 0.02%EDTA. M254/HMGS conditioned...
In order to fully explore and comprehensive utilization the resource of Dictyoploca japonica, the pupa protein was extracted form pupa of Dictyoploca japonica by sodium hydroxide in this study. The pH of the solubility of protein had been determined. The best condition of extracting pupa protein: the concentration of NaOH is 1.5%, with the temperature 70??, the time 3 h and the pupa powder should...
It's well known that irreversible cross-linked complexes between proteins, produced by formaldehyde interacting with free amino or amide groups to form Schiff s base, are formed in diabetes and aging patients. To identify such specific modified peptides as biomarkers for clinical diagnose of diabetes and AD, cross-linkage of formaldehyde with human serum albumin (HSA) was analyzed by LC/ESI/MS in...
Aligning protein sequences using a score matrix has became a routine but valuable method in modern biological research. However, alignment in "Twilight Zone" is still a challenging issue. New score matrices such as Position Specific Score Matrix and structural context-based matrix have been issued to deal with it, whereas generalized matrices are still useful in pairwise alignment and/or...
Sequence alignment has been a most commonly used method in modern biological research. However, sequence alignment in "Twilight Zone" is still a challenging issue. Three protein class-specific matrices are constructed to investigate whether alignment in "Twilight Zone" can be improved by using class-specific matrices and gap penalties. The substitution pattern differences between...
The neural stem cells were induced by low-level dopamine (DA). We allowed cells 72 h of differentiation before protein extraction. The global proteins of neural stem cells and dopaminergic neurons were extracted. In this study, the protein from cell lysat was rudiment separated by SDS-PAGE, and the gel strip was cut into several portions. The protein in each portion is digested by trypsin, and then...
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