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Background
Smith–Lemli–Opitz syndrome is a birth defect caused by the deficiency of 7‐dehydrocholesterol reductase in cholesterol biosynthesis pathway, which leads to accumulation of 7‐dehydrocholesterol and reduction of cholesterol in body fluids. To effectively diagnose Smith–Lemli–Opitz syndrome and monitor therapy, a reliable method for simultaneous detection of 7‐dehydrocholesterol and cholesterol...
Chronic exposure to manganese causes parkinsonian symptoms and has been implicated as an environmental factor in the pathogenesis of Parkinson's disease (PD). Here we show that manganese inhibits the proliferation of PC12 cells and induces apoptosis through the formation of catechol isoquinolines. Manganese induces the production of 1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline (salsolinol,...
The dopamine-derived salsolinol (Sal), 1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline, has been considered a potential endogenous neurotoxin involved in the etiology of PD. In the present study, 150 muM dopamine (DA) was applied to be the treatment concentration on account of that 150 muM DA decreased cell viability to 44.8%. The level of salsolinol in human dopaminergic SH-SY5Y cells treated...
The neural stem cells were induced by low-level dopamine (DA). We allowed cells 72 h of differentiation before protein extraction. The global proteins of neural stem cells and dopaminergic neurons were extracted. In this study, the protein from cell lysat was rudiment separated by SDS-PAGE, and the gel strip was cut into several portions. The protein in each portion is digested by trypsin, and then...
A rat model of Parkinson's disease was established by direct injection of 6-OHDA into the substantia nigra unilaterally. Animals were assessed by D-amphetamine rotation test, anti-TH staining and detection of dopamine and its metabolites by HPLC-ECD. Rotational behavior experiment shown that the achievement ratio of PD model is 48.9%. Anti-TH staining result indicated that 6-OHDA treatment caused...
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