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The ammonium‐dependent posttranslational regulation of nitrogenase activity in Azospirillum brasilense requires dinitrogenase reductase ADP‐ribosyl transferase (DraT) and dinitrogenase reductase ADP‐glycohydrolase (DraG). These enzymes are reciprocally regulated by interaction with the PII proteins, GlnB and GlnZ. In this study, purified ADP‐ribosylated Fe‐protein was used as substrate to study the...
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