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We characterized a set of Arabidopsis mutants deficient in specific light‐harvesting proteins, using freeze‐fracture electron microscopy to probe the organization of complexes in the membrane and confocal fluorescence recovery after photobleaching to probe the dynamics of thylakoid membranes within intact chloroplasts. The same methods were used to characterize mutants lacking or over‐expressing PsbS,...
Incubation of prolamellar bodies (PLB) in high-salt media leads to changes in PLB structure and properties of their protochlorophyllide oxidoreductase–protochlorophyllide (POR–PChlide) complex. The paracrystalline organisation typical of PLB is disrupted and NADPH dissociates from photoconvertible POR–PChlide, with absorption maxima at 640 and 650 nm (POR–PChlide640/650), and a non-photoconvertible...
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