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Gap junctions (GJs) are widely distributed in brains across the animal kingdom. To visualize the GJ‐ coupled networks of two major mechanosensory neurons in the ganglia of medicinal leeches, we injected these cells with the GJ‐permeable tracer Neurobiotin. When diffusion time was limited to only 30 min, tracer coupling was highly variable for both cells, suggesting a possible modulation of GJ permeability...