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In the current study, reverse‐transcription polymerase chain reaction (RT‐PCR) and rapid amplification of cDNA ends (RACE) PCR were used to clone full‐length putative Na+–H+ exchanger isoforms (NHE2a) cDNA from the gills of Fundulus heteroclitus. The 2480 bp cDNA includes a coding region for a protein that shows a 57% amino acid homology to rabbit NHE2. These sequences allowed data mining of available...
Sea water dilution (to 5% and 1% of sea water) induced a reversible, plasma metabolic acidosis in a euryhaline teleost (the oyster toadfish, Opsanus tau). Net H + transfer between the fish and the water decreased in both low salinity exposure (LSE) groups during the first week of exposure and ΔH + returned to normal after ~10 days of 5% LSE, but not during 1% LSE.
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