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As the main immunogen that could stimulate neutralized antibody in pigs, recombinant E2 protein of CSFV was expressed in CHO‐dhfr−cells driven by endogenous Txnip promoter from Chinese hamster. Different fragments of Txnip promoter were amplified by PCR from isolated genomic DNA of CHO cells and cloned into different expression vectors. Compared with CMV promoter, CHO‐pTxnip‐4‐rE2 (F12) cell clone...
A modeling method of fitting operating coils current model is presented, and the two kinds of equivalent circuit models of the operating coil current fitting are established, which are according to the two steady state two coil configuration permanent magnetic actuator of the vacuum circuit breaker, and the analysis of the relationship between the current waveform in the operating coil and the movable...
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