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MCS9.7‐lacZ expression in whole mount staining. Bgal activity was detected in transient (Rahimov et al. [2008] Nat Gen 40:1341–1347), and in stable transgenic murine embryos at E11.5. Optical tomography (OPT) movies showing the Bgal activity of stable transgenic embryo in 2‐D. Counterstaining was done using green pseudocolor and red represent Bgal expression. From Fakhouri et al., Developmental Dynamics...
Background: DNA variation in Interferon Regulatory Factor 6 (IRF6) contributes risk for orofacial clefting, including a common DNA variant rs642961. This DNA variant is located in a multi‐species conserved sequence that is 9.7 kb upstream from the IRF6 transcriptional start site (MCS9.7). The MCS9.7 element was shown to possess enhancer activity that mimicked the expression of endogenous Irf6 at embryonic...
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