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The aim of this work was to determine the relationship of intracellular reactive oxygen species (ROS) and the disulphide bonds established between sperm proteins with the achievement of capacitation in boar spermatozoa. With this purpose, spermatozoa were incubated in a specifically designed in vitro capacitation medium (CM) in the presence or absence of reduced glutathione (GSH). Incubation of boar...
The compound 2-deoxy-2-fluoro-α-d-glucopyranosyl fluoride (F2Glc), which is a nonmetabolized superior glucose analogue, is a potent inhibitor of glycogen phosphorylase and pharmacological properties are reported. Glycogen phosphorylase (GP) and glycogen synthase (GS) are responsible of the degradation and synthesis, respectively, of glycogen which is a polymer of glucose units that provides a readily...
This work analysed intracellular calcium stores of boar spermatozoa subjected to ‘in vitro’ capacitation (IVC) and subsequent progesterone‐induced acrosome exocytosis (IVAE). Intracellular calcium was analysed through two calcium markers with different physico‐chemical properties, Fluo‐3 and Rhod‐5N. Indicative parameters of IVC and IVAE were also evaluated. Fluo‐3 was located at both the midpiece...
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