Search results for: A. G. Zaraisky

Items from 1 to 18 out of 18 results

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The Mechanisms of Embryonic Scaling

A. M. Nesterenko, A. G. Zaraisky

Russian Journal of Developmental Biology > 2019 > 50 > 3 > 95-101

The phenomenon of scaling, i.e., preserving the proportions of the embryo’s spatial pattern in dependence on its overall size, is the most prominent feature of the embryonic morphogenetic fields. Up to now, attempts to understand the mechanisms of scaling have been limited to the creation and analysis of the behavior of theoretical models that, to some extent, reproduce this phenomenon in silico....

article

Lampreys, “Living Fossils,” in Research on Early Development and Regeneration in Vertebrates

A. V. Bayramov, G. V. Ermakova, A. V. Kucheryavyy, A. G. Zaraisky

Russian Journal of Developmental Biology > 2018 > 49 > 6 > 327-338

Agnathans, the most ancient of the extant vertebrates, evoke steadily increasing interest as the object of research on the basic processes of vertebrate ontogeny. Lampreys have been more accessible to researchers than myxines (hagfish), representatives of the other class of jawless vertebrates, for more than 100 years. Studies on the functional and evolutionary aspects of early ontogeny in lamprey...

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Development of Methods and Techniques to Visualize Mechanical Tension in Embryos Using Genetically Encoded Fluorescent Mechanosensors

F. M. Eroshkin, S. V. Kremnev, G. V. Ermakova, A. G. Zaraisky

Russian Journal of Developmental Biology > 2018 > 49 > 6 > 362-369

Lately, the growing body of quantitative data has provided evidence of the importance of mechanical forces in embryogenesis. The study of spatial and temporal distribution of mechanical tension in the course of embryogenesis is one of the most important problems of modern developmental biology. Development of genetically encoded fluorescent mechanosensors allowed their application in an intravital...

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Obtaining of Agr2 Specific Antibodies and Determination of the Agr2 Protein Distribution Pattern during Early Embryonic Development and Tadpole Regeneration in Xenopus laevis

A. S. Ivanova, N. Y. Martynova, P. A. Komarov, E. E. Orlov, more

Russian Journal of Developmental Biology > 2018 > 49 > 6 > 393-397

The Agr (anterior gradient) group proteins belong to the family of proteins with a noncanonical thioredoxin motif and are involved in the regulation of various processes: embryonic development, regeneration, and oncogenesis. Normally, the human Agr2 gene is predominantly expressed in the secreting cells of mucin-producing organs. However, Agr2 is associated with active metastasis during the development...

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Patterns of Mitosis and Activation of the Map-Kinase Cascade during Tadpole Tail Regeneration in the Refractory Period of Xenopus laevis Development

A. S. Ivanova, G. V. Ermakova, A. G. Zaraisky, M. B. Tereshina

Russian Journal of Developmental Biology > 2018 > 49 > 5 > 260-263

Patterns of mitotic cells’ distribution and activation of the MAP-kinase cascade during the regeneration of Xenopus laevis tadpole tails were studied before and during the refractory period. It is known that the tadpoles of Xenopus laevis are able to fully restore the full structure of the tail after amputation. However, in the refractory period (stage 45–47), the ability to regenerate is significantly...

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Molecular Mechanisms of the Xanf1 Homeobox Gene Expression Regulation during the Early Development of the Forebrain Rudiment in the Clawed Frog

F. M. Eroshkin, A. V. Bayramov, G. V. Ermakova, A. G. Zaraisky, more

Russian Journal of Bioorganic Chemistry > 2018 > 44 > 3 > 310-321

As a result of the analysis of the promoter region of the Xanf1 gene encoding the transcriptional regulator of the early development of the forebrain in the clawed frog, we found several promoter elements responsible for the expression of this gene and its localization in the anterior head region of the neural plate at the early neurula stage. A search for transcription factors that are able to bind...

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Effect of a Heterodimeric Complex of the Transcription Factors SoxD (Sox15) and Xanf1 on the Formation of the Xanf1 Gene Expression Zone during the Early Development of the Forebrain in the Spur-Toed Frog

N. Y. Martynova, F. M. Eroshkin, A. G. Zaraisky

Russian Journal of Bioorganic Chemistry > 2018 > 44 > 3 > 362-365

The effect of a protein complex consisting of the homeodomain transcription factor Xanf1 and the HMG-containing transcription factor SoxD (homologue Sox15 in human and mouse, group G of the Sox family) on the formation of a spatial pattern of the Xanf1 gene expression has been studied. The Xanf1 factor determines the early development of the forebrain, and the disturbance of its expression results...

article

Methods of In Vivo Gene-Specific Knockdown Using Morpholino and Vivo-Morpholino Oligonucleotides

A. S. Ivanova, D. D. Korotkova, N. Y. Martynova, O. V. Averyanova, more

Russian Journal of Bioorganic Chemistry > 2018 > 44 > 3 > 358-361

The functioning of the small GTPase gene, Ras-dva1, has been studied during regeneration processes of the tadpole tails of the clawed frog Xenopus laevis. For this purpose, we have developed two approaches for the gene knockdown using injections of sequence-specific antisense morpholino oligonucleotides (MO) or vivo-morpholino oligonucleotides (vivo-MO). It has been shown for the first time that intracellular...

article

Presence of homeobox gene of Anf class in Pacific lamprey Lethenteron camtschaticum confirms the hypothesis about the importance of emergence of Anf genes for the origin of telencephalon in vertebrate evolution

A. V. Bayramov, G. V. Ermakova, F. M. Eroshkin, A. V. Kucheryavyy, more

Russian Journal of Developmental Biology > 2017 > 48 > 4 > 241-251

The presence of a complex structured brain, the anterior department of which (telencephalon) has no analogues in other groups of animals, is a significant peculiarity of vertebrates (including the human). Previously, a vertebrate-specific monogenic class of homeobox genes Anf, which plays a key role in the development of telencephalon, was discovered in the Laboratory of Molecular Bases of Embryogenesis...

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Interaction of secreted factor Agr2 with its potential receptors from the family of three-finger proteins

F. M. Eroshkin, N. Y. Martynova, A. V. Bayramov, G. V. Ermakova, more

Russian Journal of Bioorganic Chemistry > 2017 > 43 > 3 > 344-346

Interactions of the secreted protein XAgr2 of the Agr (anterior gradient proteins) family with six submembrane proteins, Tfp1–6, from the family of three-finger proteins Ly6 were studied in Xenopus laevis embryos. Earlier, other authors have shown that the newt homologue of XAgr2 is able to bind the Prod1 three-finger protein, which participates in the establishment of the proximal-to-distal pattern...

article

Vizualizing the morphogen adsorption gradient in the Xenopus laevis embryo using fluorescently labeled heparin-binding motif of BMP4 morphogen

E. E. Orlov, A. M. Nesterenko, N. Y. Martynova, A. G. Zaraisky

Russian Journal of Bioorganic Chemistry > 2017 > 43 > 3 > 347-349

Spatial distribution of heparan sulfates in the embryonic extracellular matrix at midgastrula stage has been demonstrated in the Xenopus laevis embryo model. Towards this end, fluorescently labeled fusion protein EGFP-hbmBMP4 made up by green fluorescent protein (EGFP) and heparin-binding motif of Bone Morphogenetic Protein 4 (BMP4) was produced in the E. coli expression system. Xenopus laevis embryos...

article

The secreted protein Noggin4 is an activator of the Wnt/PCP-signaling pathway

A. V. Bayramov, F. M. Eroshkin, N. Y. Martynova, E. E. Orlov, more

Russian Journal of Bioorganic Chemistry > 2017 > 43 > 2 > 216-219

The protein Noggin4 of the African clawed frog Xenopus laevis has been shown to act as a modulator of the “noncanonical” Wnt/PCP-signaling pathway that plays an important role in the regulation of cell motility. Induction of disturbances in the expression of Noggin4 led to the activation of Wnt/PCP-pathway and the related anomalies of early embryonic development. The Noggin4 protein can bind the Wnt11...

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The interaction of secreted proteins Noggin4 and Wnt8 from Xenopus laevis embryos

N. Y. Martynova, A. M. Nesterenko, E. E. Orlov, F. M. Eroshkin, more

Russian Journal of Bioorganic Chemistry > 2016 > 42 > 3 > 340-342

We demonstrated that the secreted protein Noggin4 from Xenopus laevis was capable of the in vitro binding to the secreted factor Wnt8, one of the ligands of the Wnt/betaCatenin signaling pathway. It was also shown that posttranslational modifications occurring during secretion of these proteins from the embryonic cells were necessary for their effective interaction. Also, we proposed a method for...

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Secreted protein Noggin4 participates in the formation of forebrain structures in Xenopus laevis by inhibiting the Wnt/beta-catenin signaling pathway

A. V. Bayramov, F. M. Eroshkin, A. V. Borodulin, N. Yu. Martynova, more

Russian Journal of Developmental Biology > 2016 > 47 > 4 > 202-206

Noggin proteins are important regulators of the early development of the vertebrate neural system. Previously, it has been traditionally thought that vertebrates have only one noggin gene (Noggin1), whose main function is the inhibition of BMP signaling pathway during the formation of dorsoventral polarity in embryos. Then other proteins of this family were discovered, and the studies of Noggin2 protein...

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A point mutation of the Noggin2 protein increasing its binding capacity to activin

F. M. Eroshkin, N. V. Fedina, N. Y. Martynova, A. V. Bayramov, more

Russian Journal of Bioorganic Chemistry > 2015 > 41 > 6 > 675-677

Previously, we found that the Noggin family proteins could bind to activin, a member of the TGF-β superfamily, and repress the corresponding signaling cascade. In this work, we characterized a mutant of the Xenopus laevis Noggin2 protein bearing a W203R substitution. This point mutation was shown to enhance the affinity of Noggin to activin, while weakening its affinity to bone morphogenetic proteins...

article

The ag1 gene is required for the fin regeneration in Danio rerio

I. N. Shandarin, A. S. Ivanova, A. A. Minin, M. B. Tereshina, more

Russian Journal of Bioorganic Chemistry > 2015 > 41 > 4 > 379-382

—We have recently demonstrated that the Ag1 protein is necessary for the fin regeneration in Danio rerio fish. Robust activation of the ag1 gene expression in cells of the wound epithelium is observed after caudal fin amputation. Moreover, the inhibition of translation of ag1 mRNA leads to the retardation of the caudal fin regeneration. The results of our...

article

Xenopus Embryos as a Model to Study the Genetic Mechanisms of Brain Development

A. G. Zaraisky

Molecular Biology > 2004 > 38 > 1 > 34-39

The review considers the advantages of Xenopus embryos as an experimental model to study the molecular-genetic mechanisms of embryo development. The results are described that were obtained with this model in studies on the early brain development within the framework of the Russian program Human Genome.

article

A Study of a Protein of the Annexin Group in Early Fish Development. II. Determination of Complete Amino Acid Sequence

A. A. Minin, S. G. Ozerova, N. V. Khaidarova, A. G. Zaraisky

Russian Journal of Developmental Biology > 2002 > 33 > 4 > 218-220

We studied mRNA structure of 31 kDa annexin of zebrafish Brachydanio rerio using previously obtained 3"-terminal incomplete cDNA. The size of this protein mRNA was determined by Northern hybridization. PCR screening of cDNA library of zebrafish gastrula allowed us to obtain cDNA of the 5"-terminal regions of the mRNA. The primary structure of the protein deduced from the mRNA sequence allowed...

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Keywords

REGENERATION (6)
NOGGIN (4)
NOGGIN4 (3)
XENOPUS (3)
EMBRYOGENESIS (2)
LAMPREYS (2)
SOXD (2)
TELENCEPHALON DEVELOPMENT (2)
XANF1 (2)
BRACHYDANIO RERIO (1)
XENOPUS (1)
ACTIVIN (1)
AG1 (1)
AGR2 (1)
ANF (1)
ANNEXIN IV (1)
ANTERIOR GRADIENT GENES (1)
ANTIBODY PRODUCTION (1)
BMP4 (1)
BRAIN DEVELOPMENT (1)
CDNA (1)
CYCLOSTOMATA (1)
CYCLOSTOMES (1)
DANIO RERIO (1)
DEVELOPMENT (1)
DPERK1/2 (1)
EARLY EMBRYONIC DIFFERENTIATION (1)
EMBRYONIC REGULATION (1)
EMBRYOS AND TADPOLES OF XENOPUS LAEVIS (1)
EXTRACELLULAR MATRIX (1)
FORMATION OF HEAD STRUCTURES IN VERTEBRATES (1)
FUNCTIONAL GENOMICS (1)
GALLUS (1)
GENE EXPRESSION (1)
IN SITU HYBRIDIZATION (1)
LANF (1)
LETHENTERON CAMTSCHATICUM (1)
MAP-KINASE CASCADE (1)
MECHANIСAL TENSION (1)
MECHANOSENSORS (1)
MITOSES (1)
MORPHOGEN GRADIENTS (1)
MORPHOGENETIC FIELDS (1)
MORPHOLINO AND VIVO-MORPHOLINO OLIGONUCLEOTIDES (1)
MRNA (1)
MUTATION (1)
NEURAL INDUCTION (1)
NOGGIN1 (1)
NOGGIN2 (1)
ONE-HYBRID SYSTEM (1)
OTX2 (1)
RAS-DVA SMALL GTPASES (1)
REFRACTORY PERIOD (1)
REGENERATIVE MARKER GENE FGF20 (1)
SCALING (1)
TADPOLES OF XENOPUS LAEVIS (1)
TFP4 (1)
TGF-Β (1)
TRANSCRIPTION FACTORS (1)
WNT/BETA-CATENIN SIGNALING PATHWAY (1)
WNT/PCP (1)
WNT11 (1)
WNT5A (1)
WNT8 (1)
XAGR2 (1)
XENOPUS LAEVIS TADPOLES (1)
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