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In the present investigation, dilute sulphuric acid pretreatment conditions were optimized for Saccharum spontaneum through Box–Bhenken design of response surface methodology for cellulase production. Cellulase production was carried out in 250 ml capacity Erlenmeyer flask using pretreated S. spontaneum as substrate by Bacillus subtilis K-18 incubated at 50 °C for 24 h of fermentation period. Results...