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We developed new F1 hybrids of Chinese cabbage (Brassica rapa L. ssp. pekinensis) that allow cultivation earlier in spring without heating by introducing extremely late-bolting alleles at two homologs of the flowering repressor Flowering Locus C (BrFLC2 and BrFLC3) from non-heading ‘Leafy Green Parental Line No. 2’. These new F1 hybrids were produced by the following four steps. First, the extremely...
The multiplex reverse transcription polymerase chain reaction (RT-PCR) was used to detect Broad bean wilt virus 2, Cucumber mosaic virus and Clover yellow vein virus. Using specific primers, the method enabled detection of the viruses with a high degree of sensitivity and specificity and identification of the virus species according to the size of the PCR product amplified. Each of the three viruses...
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