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In this work, we fabricate a surface-enhanced Raman scattering (SERS)-active substrate with low background signals using bottom-up approach. Staphylococcus aureus has been used as Raman probe to evaluate its enhancement capability. The experimental results of this study show that Staphylococcus aureus was well resolved using gold-coated hydrothermally roughened TiO2 substrates.
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