Search results for: Haleema Janjua

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article

The RAS-Binding Domain of Human BRAF Protein Serine/Threonine Kinase Exhibits Allosteric Conformational Changes upon Binding HRAS

James M. Aramini, Sergey M. Vorobiev, Lynda M. Tuberty, Haleema Janjua, more

Structure > 2015 > 23 > 8 > 1382-1393

RAS binding is a critical step in the activation of BRAF protein serine/threonine kinase and stimulation of the mitogen-activated protein kinase signaling pathway. Mutations in both RAS and BRAF are associated with many human cancers. Here, we report the solution nuclear magnetic resonance (NMR) and X-ray crystal structures of the RAS-binding domain (RBD) from human BRAF. We further studied the complex...

article

A General Computational Approach for Repeat Protein Design

Fabio Parmeggiani, Po-Ssu Huang, Sergey Vorobiev, Rong Xiao, more

Journal of Molecular Biology > 2015 > 427 > 2 > 563-575

Repeat proteins have considerable potential for use as modular binding reagents or biomaterials in biomedical and nanotechnology applications. Here we describe a general computational method for building idealized repeats that integrates available family sequences and structural information with Rosetta de novo protein design calculations. Idealized designs from six different repeat families were...

article

Solution NMR structure of Alr2454 from Nostoc sp. PCC 7120, the first structural representative of Pfam domain family PF11267

James M. Aramini, Donald Petrey, Dong Yup Lee, Haleema Janjua, more

Journal of Structural and Functional Genomics > 2012 > 13 > 3 > 171-176

Protein domain family PF11267 (DUF3067) is a family of proteins of unknown function found in both bacteria and eukaryotes. Here we present the solution NMR structure of the 102-residue Alr2454 protein from Nostoc sp. PCC 7120, which constitutes the first structural representative from this conserved protein domain family. The structure of Nostoc sp. Alr2454 adopts a novel protein fold.

article

A large conformational change in the putative ATP pyrophosphatase PF0828 induced by ATP binding

Farhad Forouhar, Nabila Saadat, Munif Hussain, Jayaraman Seetharaman, more

Acta Crystallographica Section F > 67 > 11 > 1323 - 1327

ATP pyrophosphatases (ATP PPases) are widely distributed in archaea and eukaryotes. They share an HUP domain at the N‐terminus with a conserved PP‐motif that interacts with the phosphates of ATP. The PF0828 protein from Pyrococcus furiosus is a member of the ATP PPase superfamily and it also has a 100‐residue C‐terminal extension that contains a strictly conserved EGG(E/D)xE(T/S) motif, which has...

article

Solution NMR structure of Dsy0195 homodimer from Desulfitobacterium hafniense: first structure representative of the YabP domain family of proteins involved in spore coat assembly

Yunhuang Yang, Theresa A. Ramelot, John R. Cort, Huang Wang, more

Journal of Structural and Functional Genomics > 2011 > 12 > 3 > 175-179

Protein domain family YabP (PF07873) is a family of small protein domains that are conserved in a wide range of bacteria and involved in spore coat assembly during the process of sporulation. The 62-residue fragment of Dsy0195 from Desulfitobacterium hafniense, which belongs to the YabP family, exists as a homodimer in solution under the conditions used for structure determination using NMR spectroscopy...

article

Solution NMR and X-ray crystal structures of membrane-associated Lipoprotein-17 domain reveal a novel fold

Rajeswari Mani, Sergey Vorobiev, G. V. T. Swapna, Helen Neely, more

Journal of Structural and Functional Genomics > 2011 > 12 > 1 > 27-32

The conserved Lipoprotein-17 domain of membrane-associated protein Q9PRA0_UREPA from Ureaplasma parvum was selected for structure determination by the Northeast Structural Genomics Consortium, as part of the Protein Structure Initiative’s program on structure–function analysis of protein domains from large domain sequence families lacking structural representatives. The 100-residue Lipoprotein-17...

article

The high-throughput protein sample production platform of the Northeast Structural Genomics Consortium

Rong Xiao, Stephen Anderson, James Aramini, Rachel Belote, more

Journal of Structural Biology > 2010 > 172 > 1 > 21-33

We describe the core Protein Production Platform of the Northeast Structural Genomics Consortium (NESG) and outline the strategies used for producing high-quality protein samples. The platform is centered on the cloning, expression and purification of 6X-His-tagged proteins using T7-based Escherichia coli systems. The 6X-His tag allows for similar purification procedures for most targets and implementation...

article

Combining NMR and EPR Methods for Homodimer Protein Structure Determination

Yunhuang Yang, Theresa A. Ramelot, Robert M. McCarrick, Shuisong Ni, more

Journal of the American Chemical Society > 2010 > 132 > 34 > 11910 - 11913

article

Solution NMR structure of Lin0431 protein from Listeria innocua reveals high structural similarity with domain II of bacterial transcription antitermination protein NusG

Yuefeng Tang, Rong Xiao, Colleen Ciccosanti, Haleema Janjua, more

Proteins: Structure, Function, and Bioinformatics > 78 > 11 > 2563 - 2568

article

Functional insights from structural genomics

Farhad Forouhar, Alexandre Kuzin, Jayaraman Seetharaman, Insun Lee, more

Journal of Structural and Functional Genomics > 2007 > 8 > 2-3 > 37-44

Structural genomics efforts have produced structural information, either directly or by modeling, for thousands of proteins over the past few years. While many of these proteins have known functions, a large percentage of them have not been characterized at the functional level. The structural information has provided valuable functional insights on some of these proteins, through careful structural...

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Keywords

STRUCTURAL GENOMICS (5)
NMR (2)
PROTEIN STRUCTURE INITIATIVE (2)
UREAPLASMA PARVUM (1)
6X-HIS TAG (1)
ACETYLTRANSFERASE (1)
ALR2454 PROTEIN (1)
ATP BINDING (1)
ATP PYROPHOSPHATASES (1)
COMPUTATIONAL DESIGN (1)
CONSTRUCT OPTIMIZATION (1)
DE NOVO DESIGN (1)
DISORDER PREDICTION (1)
DOMAINS OF UNKNOWN FUNCTION (DUFS) (1)
DSY0195 (1)
DUF3067 (1)
HDX-MS (1)
HIGH-THROUGHPUT PROTEIN PRODUCTION (1)
IDEALIZED PROTEINS (1)
LABORATORY INFORMATION MANAGEMENT SYSTEM (1)
LIGATION-INDEPENDENT CLONING (1)
LIN0431 (1)
LIPOPROTEIN (1)
LIPOPROTEIN-17 DOMAIN OF Q9PRA0 UREPA (1)
METHYLTRANSFERASE (1)
MULTIPLE DISPLACEMENT AMPLIFICATION (1)
NMR MICROPROBE SCREENING (1)
NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG) (1)
NUSG (1)
PARALLEL PROTEIN PURIFICATION (1)
PF07873 (1)
PF0828 (1)
PF11267 (1)
PYROCOCCUS FURIOSUS (1)
REPEAT PROTEINS (1)
SOLUTION NMR (1)
SOLUTION NMR STRUCTURE (1)
SPERMINE (1)
SPORULATION PROTEIN (1)
T7 ESCHERICHIA COLI EXPRESSION SYSTEM (1)
THERMODYNAMIC STABILITY (1)
WHEAT GERM CELL-FREE (1)
X-RAY CRYSTAL STRUCTURE (1)
X-RAY CRYSTALLOGRAPHY (1)
YABP (1)
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Springer (4)
Elsevier (3)
Wiley (2)
ACS (1)

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Search results for: Haleema Janjua

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