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AbstractWe describe two modifications of the popular reporter green fluorescent protein (GFP) which have short half-lives in our system, the cellular slime mould Dictyosteliumdiscoideum. One of these bears an N-terminal ubiquitin; this GFP was originally planned to be a substrate of the N-end-rule pathway, but deubiquitination does not seem to occur, and a degradation by the UFD (ubiquitin-fusion-degradation...