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A submergence-induced gene, OsGGT, was cloned from 7-day submerged rice (Oryza sativa L. plants, FR13A (a submergence-tolerant cultivar, Indica), using suppression subtractive hybridization and both 5′- and 3′-rapid amplification of cDNA ends (RACE). The full-length OsGGT cDNA contains 1,273 bp with an open reading frame of 1,140 bp (17–1,156) that encodes 379 amino acids. Its deduced amino acid sequence...
We used suppression subtractive hybridization (SSH) to identify submergence-induced genes from the submergence-tolerant rice cultivar, FR13A. These genes putatively encode four proteins including a cation transport protein (OsCTP), monogalactosyl–diacylglycerol synthase, a cold-induced protein and glutathione synthetase. The full-length gene of one upregulated clone, OsCTP was generated by rapid amplification...
Suppression subtractive hybridization was used to construct a subtractive cDNA library from plants of non-submerged and 7-day-submerged rice (Oryza sativa L., FR13A, a submergence-tolerant cultivar). One clone of the subtractive cDNA library, S23, was expressed abundantly during submergence. The full length of S23 was amplified using 5- and 3-rapid amplification of cDNA ends, and found to consist...
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