Search results for: Abdelkader Namane

Items from 1 to 20 out of 21 results

article

Differential expression of Ixodes ricinus salivary gland proteins in the presence of the Borrelia burgdorferi sensu lato complex

Violaine Cotté, Laurence Sabatier, Gilles Schnell, Annick Carmi-Leroy, more

Journal of Proteomics > 2014 > 96 > Complete > 29-43

In Europe, Ixodes ricinus is the main vector of Lyme borreliosis. Their salivary glands play a critical role in the biological success of ticks. To better understand the cross-talk between Borrelia burgdorferi and tick salivary glands, we analyzed protein expression in the salivary glands of I. ricinus adult ticks that were infected by various strains of the B. burgdorferi sl complex. iTRAQ allowed...

article

Isolation, Functional Characterization and Proteomic Identification of CC2-PLA₂ from Cerastes cerastes Venom: A Basic Platelet-Aggregation-Inhibiting Factor

Fatah Chérifi, Abdelkader Namane, Fatima Laraba-Djebari

The Protein Journal > 2014 > 33 > 1 > 61-74

Three-step chromatography and proteomic analysis have been used to purify and characterize a new basic phospholipase A₂ named CC2-PLA₂ from the venom of Cerastes cerastes. This phospholipase A₂ has been isolated to an extent of about 50-folds and its molecular weight was estimated at 13,534 Da. For CC2-PLA₂ identification and LC-MALDI-MS/MS analysis, the protein was reduced, alkylated and double hydrolyzed...

article

Use and recycling of Ca-alginate biocatalyst for removal of phenol from wastewater

Oumessaad Ali, Abdelkader Namane, Amina Hellal

Journal of Industrial and Engineering Chemistry > 2013 > 19 > 4 > 1384-1390

The objective of the current work is the exhaustive study of the phenol degradation potential in both free cell and immobilized bacterium (Pseudomonas aeruginosa) in calcium alginate beads (biocatalyst) was investigated for its ability to grow and degrade phenol as its sole source of carbon and energy.The biodegradation assays were performed in liquid medium with phenol being the only substrate. It...

article

Identification of Outer Membrane Proteins Altered in Response to UVC-Radiation in Vibrio parahaemolyticus and Vibrio alginolyticus

Fethi Ben Abdallah, Rihab Lagha, Ali Ellafi, Abdelkader Namane, more

Indian Journal of Microbiology > 2012 > 52 > 4 > 660-665

Vibrio parahaemolyticus and V. alginolyticus, marine foodborne pathogens, were treated with UVC-radiation (240 J/m²) to evaluate alterations in their outer membrane protein profiles. Outer membrane protein patterns of UVC-irradiated bacteria were found altered when analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Altered proteins were identified by mass spectrometry (MS and...

article

A Critical Role of Perinuclear Filamentous Actin in Spatial Repositioning and Mutually Exclusive Expression of Virulence Genes in Malaria Parasites

Qingfeng Zhang, Yufu Huang, Yilong Zhang, Xiaonan Fang, more

Cell Host & Microbe > 2011 > 10 > 5 > 451-463

Many microbial pathogens, including the malaria parasite Plasmodium falciparum, vary surface protein expression to evade host immune responses. P. falciparium antigenic variation is linked to var gene family-encoded clonally variant surface protein expression. Mututally exclusive var gene expression is partially controlled by spatial positioning; silent genes are retained at distinct perinuclear sites...

article

Identification of outer membrane proteins of Vibrio parahaemolyticus and Vibrio alginolyticus altered in response to γ-irradiation or long-term starvation

Fethi Ben Abdallah, Ali Ellafi, Rihab Lagha, Amina Bakhrouf, more

Research in Microbiology > 2010 > 161 > 10 > 869-875

Vibrio parahaemolyticus and Vibrio alginolyticus were subjected to γ-irradiation (0.5 kGy) or starvation by incubation for 8 months in seawater to study modifications in their outer membrane protein patterns. After treatment, outer membrane protein profiles of starved or γ-irradiated bacteria were found to be altered when analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)...

article

Identification of Leishmania‐specific protein phosphorylation sites by LC‐ESI‐MS/MS and comparative genomics analyses

Sonia Hem, Pier Federico Gherardini, José Osorio y Fortéa, Veronique Hourdel, more

PROTEOMICS > 10 > 21 > 3868 - 3883

Human pathogenic protozoa of the genus Leishmania undergo various developmental transitions during the infectious cycle that are triggered by changes in the host environment. How these parasites sense, transduce, and respond to these signals is only poorly understood. Here we used phosphoproteomic approaches to monitor signaling events in L. donovani axenic amastigotes, which may be important for...

article

CCSV-MPase, a Novel Procoagulant Metalloproteinase from Cerastes cerastes Venom: Purification, Biochemical Characterization and Protein Identification

Fatah Chérifi, Jean-Claude Rousselle, Abdelkader Namane, Fatima Laraba-Djebari

The Protein Journal > 2010 > 29 > 7 > 466-474

A procoagulant metalloproteinase called CCSV-MPase was purified from C. cerastes venom by successive chromatographic methods starting with gel-filtration through Sephadex G-75; ion-exchange DEAE-Cellulose A-50; affinity chromatography on Benzamidine Sepharose 6B and RP-HPLC on a C8 column. CCSV-MPase has been isolated to an extent of about tenfolds and its molecular mass was evaluated at 70 kDa by...

article

Deletion of EFL1 Results in Heterogeneity of the 60S GTPase-associated rRNA Conformation

Jean-Sébastien Graindorge, Jean-Claude Rousselle, Bruno Senger, Pascal Lenormand, more

Journal of Molecular Biology > 2005 > 352 > 2 > 355-369

Previous work suggested that the release of the nucleolar Tif6 from nascent 60S subunits occurs in the cytoplasm and requires the cytoplasmic EF-2-like GTPase, Efl1. To check whether this release involves an rRNA structural rearrangement mediated by Efl1, we analyzed the rRNA conformation of the GTPase center of 80S ribosomes in three contexts: wild-type, Δefl1 and a dominant suppressor R1 of Δefl1...

article

Limited Efficiency of Prolyl-Endopeptidase in the Detoxification of Gliadin Peptides in Celiac Disease

Tamara Matysiak–Budnik, Celine Candalh, Christophe Cellier, Christophe Dugave, more

Gastroenterology > 2005 > 129 > 3 > 786-796

Background & Aims: The resistance of prolamines to digestive enzymes is thought to be a key contributor to the pathogenesis of celiac disease by promoting the intestinal entrance of peptides able to trigger inflammation in at-risk individuals. Oral administration of a bacterial prolyl-endopeptidase (PEP) therefore was proposed as a treatment for celiac disease. To delineate the feasibility of...

article

Cryptic Pol II Transcripts Are Degraded by a Nuclear Quality Control Pathway Involving a New Poly(A) Polymerase

Françoise Wyers, Mathieu Rougemaille, Gwenaël Badis, Jean-Claude Rousselle, more

Cell > 2005 > 121 > 5 > 725-737

Since detection of an RNA molecule is the major criterion to define transcriptional activity, the fraction of the genome that is expressed is generally considered to parallel the complexity of the transcriptome. We show here that several supposedly silent intergenic regions in the genome of S. cerevisiae are actually transcribed by RNA polymerase II, suggesting that the expressed fraction of the genome...

article

LANCL1, an erythrocyte protein recruited to the Maurer's clefts during Plasmodium falciparum development

Thierry Blisnick, Laetitia Vincensini, Jean Christophe Barale, Abdelkader Namane, more

Molecular & Biochemical Parasitology > 2005 > 141 > 1 > 39-47

As the malarial parasite Plasmodium falciparum develops inside the erythrocyte, parasite-derived membrane structures, referred to as Maurer's clefts, play an important role in parasite development by delivering parasite proteins to the host cell surface, and participating in the assembly of the cytoadherence complex, essential for the pathogenesis of cerebral malaria. PfSBP1 is an integral membrane...

article

Plasmin cleaves the juxtamembrane domain and releases truncated species of the urokinase receptor (CD87) from human bronchial epithelial cells

Nathalie Beaufort, Dominique Leduc, Jean-Claude Rousselle, Abdelkader Namane, more

FEBS Letters > 2004 > 574 > 1-3 > 89-94

The three-domain (D1D2D3) urokinase receptor (CD87) is highly susceptible to cleavage within the D1-D2 linker sequence, but also within the juxtamembrane region by yet poorly characterized proteinases, allowing the release of D1 and D2D3 species in various (patho)physiological body fluids. Using immunoblot analysis and ELISA applied to a recombinant soluble CD87 and to CD87-expressing epithelial cells,...

article

Alterations of the intestinal transport and processing of gliadin peptides in celiac disease

Tamara Matysiak-Budnik, Celine Candalh, Christophe Dugave, Abdelkader Namane, more

Gastroenterology > 2003 > 125 > 3 > 696-707

Background & aims:The hypothesis of a defect in the intestinal transport and processing of toxic (31–49) or immunostimulant (57–68 and the 33-mer 56–89) gliadin peptides was tested in patients with active celiac disease (ACD), patients with treated celiac disease (TCD), and controls. Methods: Using duodenal biopsy specimens mounted in Ussing chambers, we measured electrical resistance, mucosal-to-serosal...

article

A proteomic analysis of the salt stress response of Listeria monocytogenes

Ophélie Duché, Frédéric Trémoulet, Abdelkader Namane, The European Listeria Genome Consortium, more

FEMS Microbiology Letters > 2002 > 215 > 2 > 183-188

Protein variations in Listeria monocytogenes were analyzed by 2-D electrophoresis. Bacteria were grown either in a rich medium or in a chemically defined medium. Three proteins, which are more expressed in the chemically defined medium than in the rich medium, were identified by mass spectrometry. They are closely related to AppA, Ctc and YvyD. After an osmotic shock, according to the medium and the...

article

A proteomic study of Escherichia coli O157:H7 NCTC 12900 cultivated in biofilm or in planktonic growth mode

Frédéric Trémoulet, Ophélie Duché, Abdelkader Namane, Brigitte Martinie, more

FEMS Microbiology Letters > 2002 > 215 > 1 > 7-14

Escherichia coli 0157:H7 biofilms were studied by a new method of cultivation in order to identify some of the proteins involved in the biofilm phenotype. A proteomic analysis of sessile or planktonic bacteria of the same age was carried out by two-dimensional electrophoresis, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) and database searching. Comparison...

article

Protein sequencing and identification using tandem mass spectrometry. Edited by Michael Kinter, Nicholas E. Sherman, published by Wiley-Interscience Series on Mass Spectrometry, 2000, 301 p.

Abdelkader Namane

Biochimie > 2002 > 84 > 4 > 351-352

article

A yeast homolog of chromatin assembly factor 1 is involved in early ribosome assembly

Sigrid Schaper, Micheline Fromont-Racine, Patrick Linder, Jesús de la Cruz, more

Current Biology > 2001 > 11 > 23 > 1885-1890

Cells have a recurrent need for the correct assembly of protein-nucleic acid complexes. We have studied a yeast homolog of the smallest subunit of chromatin assembly factor 1 (CAF1), encoded by YMR131c and termed “RRB1” [1]. Unlike other yeast homologs, Msi1p, and Hat2p, Rrb1p is essential for cell viability. Impairment of Rrb1p function results in decreased levels of free 60S ribosomal subunits and...

article

Assay of Nucleoside 2-Deoxyribosyltransferase Activity with Pyruvate Kinase/Lactate Dehydrogenase Coupling System

Elisabeth Pistotnik, Hiroshi Sakamoto, Sylvie Pochet, Abdelkader Namane, more

Analytical Biochemistry > 1999 > 271 > 2 > 192-193

article

Synthesis of Acylphosphates of Purine Ribonucleosides

Annett Kreimeyer, Joël Ughetto-Monfrin, Abdelkader Namane, Tam Huynh-Dinh

Tetrahedron Letters > 1996 > 37 > 48 > 8739-8742

Nucleotides do not penetrate cells at a sufficient rate to realize their therapeutic potential. To overcome this limitation we have envisaged acyl nucleodi(tri)phosphates (ND(T)Ps) as suitable membrane permeable prodrugs because a) preliminary experiences have shown that these compounds are preferably cleaved at their mixed carboxylic phosphoric bond to generate the corresponding carboxylic group...

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Search results for: Abdelkader Namane

Differential expression of Ixodes ricinus salivary gland proteins in the presence of the Borrelia burgdorferi sensu lato complex

Isolation, Functional Characterization and Proteomic Identification of CC2-PLA₂ from Cerastes cerastes Venom: A Basic Platelet-Aggregation-Inhibiting Factor

Use and recycling of Ca-alginate biocatalyst for removal of phenol from wastewater

Identification of Outer Membrane Proteins Altered in Response to UVC-Radiation in Vibrio parahaemolyticus and Vibrio alginolyticus

A Critical Role of Perinuclear Filamentous Actin in Spatial Repositioning and Mutually Exclusive Expression of Virulence Genes in Malaria Parasites

Identification of outer membrane proteins of Vibrio parahaemolyticus and Vibrio alginolyticus altered in response to γ-irradiation or long-term starvation

Identification of Leishmania‐specific protein phosphorylation sites by LC‐ESI‐MS/MS and comparative genomics analyses

CCSV-MPase, a Novel Procoagulant Metalloproteinase from Cerastes cerastes Venom: Purification, Biochemical Characterization and Protein Identification

Deletion of EFL1 Results in Heterogeneity of the 60S GTPase-associated rRNA Conformation

Limited Efficiency of Prolyl-Endopeptidase in the Detoxification of Gliadin Peptides in Celiac Disease

Cryptic Pol II Transcripts Are Degraded by a Nuclear Quality Control Pathway Involving a New Poly(A) Polymerase

LANCL1, an erythrocyte protein recruited to the Maurer's clefts during Plasmodium falciparum development

Plasmin cleaves the juxtamembrane domain and releases truncated species of the urokinase receptor (CD87) from human bronchial epithelial cells

Alterations of the intestinal transport and processing of gliadin peptides in celiac disease

A proteomic analysis of the salt stress response of Listeria monocytogenes

A proteomic study of Escherichia coli O157:H7 NCTC 12900 cultivated in biofilm or in planktonic growth mode

Protein sequencing and identification using tandem mass spectrometry. Edited by Michael Kinter, Nicholas E. Sherman, published by Wiley-Interscience Series on Mass Spectrometry, 2000, 301 p.

A yeast homolog of chromatin assembly factor 1 is involved in early ribosome assembly

Assay of Nucleoside 2-Deoxyribosyltransferase Activity with Pyruvate Kinase/Lactate Dehydrogenase Coupling System

Synthesis of Acylphosphates of Purine Ribonucleosides

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