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AbstractTo facilitate construction of physical map of the rice genome, a bacterial artificial chromosome (BAC) library of IR64 genomic DNA was constructed. It consists of 18432 clones and contains 3.28 rice genomic equivalents. The insert size ranged from 37 to 364kb with an average of 107kb. We used 31 RFLP markers on chromosome 4 to screen the library by colony hybridization. Sixty eight positive...