Search results for: Kwai-Lin Thong

Items from 1 to 20 out of 56 results

article

Molecular characterization of Vibrio cholerae O1 El Tor strains in Malaysia revealed genetically diverse variant lineages

Kwai Lin Thong, Kathryn Bee Lin Tham, Soo Tein Ngoi, Shiang Chiet Tan, more

Transboundary and Emerging Diseases > 69 > 4 > e693 - e703

Vibrio cholerae O1 El Tor variants have been the major causative agents of cholera worldwide since their emergence in the 2000s. Cholera remains endemic in some regions in Malaysia. Therefore, we aimed to investigate the genetic characteristics of the V. cholerae O1 El Tor strains associated with outbreaks and sporadic cases to elucidate the molecular evolution among the strains circulating in this...

article

Pre-enrichment step, incubation temperature and type of selective media affect the pathogenic Vibrio parahaemolyticus detection efficiency in frozen prawns

Salima Sadeghi, Kwai Lin Thong, Lay Ching Chai

Journal of Consumer Protection and Food Safety > 2019 > 14 > 4 > 355-364

This study evaluates the performance of different detection protocols of Vibrio parahaemolyticus in frozen prawns. The effect of enrichment and incubation temperature on the selectivity of thiosulfate citrate bile sucrose (TCBS) and CHROMagar™ Vibrio (CAV) media were determined. Enumeration of V. parahaemolyticus in frozen prawns by direct-plating on CAV at 37 °C yielded the highest count compared...

article

Role of coaggregation in the pathogenicity and prolonged colonisation of Vibrio cholerae

Yien Shin Toh, Soo Ling Yeoh, Ivan Kok Seng Yap, Cindy Shuan Ju Teh, more

Medical Microbiology and Immunology > 2019 > 208 > 6 > 793-809

Cholera is an acute diarrheal illness caused by the Gram-negative bacterium Vibrio cholerae. The pathogen is known for its ability to form biofilm that confers protection against harsh environmental condition and as part of the colonisation process during infection. Coaggregation is a process that facilitates the formation of biofilm. In a preliminary in vitro study, high coaggregation index and biofilm...

article

Catabolic activity and biofilm formation of foodborne Listeria monocytogenes strains

Nowshin Shyara Sharar, Lay Ching Chai, Kwai Lin Thong

Journal of Consumer Protection and Food Safety > 2018 > 13 > 3 > 289-298

Listeria monocytogenes is a major foodborne pathogen causing increased morbidity worldwide. It forms resistant biofilm structures in food processing facilities after sanitization, consequently creating a public health concern. Many studies on the metabolism and transmission of L. monocytogenes has provided insights into its intracellular infection process, however there is limited understanding on...

article

Treatment of Cotton by β-Cyclodextrin/Triclosan Inclusion Complex and Factors Affecting Antimicrobial Properties

Mikhail Novikov, Kwai Lin Thong, Nur Izzurianna Mohd Zazali, Sharifah Bee Abd Hamid

Fibers and Polymers > 2018 > 19 > 3 > 548-560

The efficacy of antimicrobial treatment of cotton fabrics depends on various parameters of the coating process, such as the chemical nature and concentration of the antimicrobial agent, the composition of the crosslinking formulation, and the curing temperature. The inclusion complex of triclosan with β-cyclodextrin (βCD) was synthesized and characterized by FTIR, XRD, NMR, Raman, SEM, and TGA. The...

article

A microdevice for rapid, monoplex and colorimetric detection of foodborne pathogens using a centrifugal microfluidic platform

Abkar Sayad, Fatimah Ibrahim, Shah Mukim Uddin, Jongman Cho, more

Biosensors and Bioelectronics > 2018 > 100 > C > 96-104

Outbreaks of foodborne diseases have become a global health concern; hence, many improvements and developments have been made to reduce the risk of food contamination. We developed a centrifugal microfluidic automatic wireless endpoint detection system integrated with loop mediated isothermal amplification (LAMP) for monoplex pathogen detection. Six identical sets were designed on the microfluidic...

article

Development of an aptasensor using reduced graphene oxide chitosan complex to detect Salmonella

Ignatius Julian Dinshaw, Shalini Muniandy, Swe Jyan Teh, Fatimah Ibrahim, more

Journal of Electroanalytical Chemistry > 2017 > 806 > C > 88-96

The development of accurate and rapid biosensors to detect pathogenic Salmonella enterica is an active area of interest with significant impact towards public health. An electrochemical aptasensor was developed using electrochemically-reduced graphene oxide-chitosan (rGO-CHI) composite as a conductive substrate to detect whole-cell Salmonella enterica serovar Typhimurium, a common serovar that causes...

article

Genetic diversity, virulotyping and antimicrobial resistance susceptibility of Yersinia enterocolitica isolated from pigs and porcine products in Malaysia

Kwai Lin Thong, Lai Kuan Tan, Peck Toung Ooi

Journal of the Science of Food and Agriculture > 98 > 1 > 87 - 95

BACKGROUND The objectives of the present study were to determine the antimicrobial resistance, virulotypes and genetic diversity of Yersinia enterocolitica isolated from uncooked porcine food and live pigs in Malaysia. RESULTS Thirty‐two non‐repeat Y. enterocolitica strains of three bioserotypes (3 variant/O:3, n = 27; 1B/O:8, n = 3; 1A/O:5, n = 2) were analysed. Approximately 90% of strains were...

article

Graphene-based label-free electrochemical aptasensor for rapid and sensitive detection of foodborne pathogen

Shalini Muniandy, Ignatius Julian Dinshaw, Swe Jyan Teh, Chin Wei Lai, more

Analytical and Bioanalytical Chemistry > 2017 > 409 > 29 > 6893-6905

Reduced graphene oxide (rGO) has emerged as a promising nanomaterial for reliable detection of pathogenic bacteria due to its exceptional properties such as ultrahigh electron transfer ability, large surface to volume ratio, biocompatibility, and its unique interactions with DNA bases of the aptamer. In this study, rGO-azophloxine (AP) nanocomposite aptasensor was developed for a sensitive, rapid,...

article

Diversity of microbiota associated with symptomatic and non-symptomatic bacterial wilt-diseased banana plants determined using 16S rRNA metagenome sequencing

Nurul Shamsinah Mohd Suhaimi, Share-Yuan Goh, Noni Ajam, Rofina Yasmin Othman, more

World Journal of Microbiology and Biotechnology > 2017 > 33 > 9 > 1-10

Banana is one of the most important fruits cultivated in Malaysia, and it provides many health benefits. However, bacterial wilt disease, which attacks bananas, inflicts major losses on the banana industry in Malaysia. To understand the complex interactions of the microbiota of bacterial wilt-diseased banana plants, we first determined the bacterial communities residing in the pseudostems of infected...

article

Salmonella Typhi genomics: envisaging the future of typhoid eradication

Kien‐Pong Yap, Kwai Lin Thong

Tropical Medicine & International Health > 22 > 8 > 918 - 925

Next‐generation whole‐genome sequencing has revolutionised the study of infectious diseases in recent years. The availability of genome sequences and its understanding have transformed the field of molecular microbiology, epidemiology, infection treatments and vaccine developments. We review the key findings of the publicly accessible genomes of Salmonella enterica serovar Typhi since the first complete...

article

Prevalence of methicillin-resistant Staphylococcus aureus (MRSA) infection and the molecular characteristics of MRSA bacteraemia over a two-year period in a tertiary teaching hospital in Malaysia

Pik San Sit, Cindy Shuan Ju Teh, Nuryana Idris, I-Ching Sam, more

BMC Infectious Diseases > 2017 > 17 > 1 > 1-14

Background Methicillin-resistant Staphylococcus aureus (MRSA) is an established pathogen that causes hospital- and community-acquired infections worldwide. The prevalence rate of MRSA infections were reported to be the highest in Asia. As there is limited epidemiological study being done in Malaysia, this study aimed to determine the prevalence of MRSA infection and the molecular characteristics of...

article

Comparative genome analysis of multiple vancomycin-resistant Enterococcus faecium isolated from two fatal cases

Shu Yong Lim, Kien-Pong Yap, Cindy Shuan Ju Teh, Kartini Abdul Jabar, more

Infection, Genetics and Evolution > 2017 > 49 > C > 55-65

Enterococcus faecium is both a commensal of the human intestinal tract and an opportunistic pathogen. The increasing incidence of enterococcal infections is mainly due to the ability of this organism to develop resistance to multiple antibiotics, including vancomycin. The aim of this study was to perform comparative genome analyses on four vancomycin-resistant Enterococcus faecium (VRE_fm) strains...

article

Comparative genomics analyses revealed two virulent Listeria monocytogenes strains isolated from ready-to-eat food

Shu Yong Lim, Kien-Pong Yap, Kwai Lin Thong

Gut Pathogens > 2016 > 8 > 1 > 1-8

Background Listeria monocytogenes is an important foodborne pathogen that causes considerable morbidity in humans with high mortality rates. In this study, we have sequenced the genomes and performed comparative genomics analyses on two strains, LM115 and LM41, isolated from ready-to-eat food in Malaysia. Results The genome size of LM115 and LM41 was 2,959,041 and 2,963,111 bp, respectively. These...

article

Evaluation of basic knowledge on food safety and food handling practices amongst migrant food handlers in Peninsular Malaysia

Pei Yee Woh, Kwai Lin Thong, Jerzy Marian Behnke, John Watkin Lewis, more

Food Control > 2016 > 70 > C > 64-73

This study was aimed to measure the basic knowledge on food safety and food handling practices among migrant food handlers as these information is scarce in Malaysia. A cross-sectional study was conducted face-to-face amongst 383 migrant food handlers from three major cities in Peninsular Malaysia through questionnaire. Socio-demographic information of all respondents was collected. Questions on food...

article

Antagonistic effects of biofilm-forming bacterial strains co-inoculated with blood disease pathogenic strain, Ralstonia syzygii subspecies celebesensis in banana plants

Nurul Shamsinah Mohd Suhaimi, Rozeita Laboh, Noni Ajam, Kwai Lin Thong

European Journal of Plant Pathology > 2017 > 148 > 1 > 13-26

A blood disease pathogenic strain, Ralstonia syzygii subspecies celebesensis was used to study the possible association of biofilm-forming bacteria with the development and severity of blood disease in banana plants. Therefore, the objective of this study was to determine the effects of mono-culture and co-culture inoculation of isolated biofilm-forming bacteria with the blood disease pathogen in...

article

Physio-chemical, microbiological properties of tempoyak and molecular characterisation of lactic acid bacteria isolated from tempoyak

Li-Oon Chuah, Ahamed Kamal Shamila-Syuhada, Min Tze Liong, Ahmad Rosma, more

Food Microbiology > 2016 > 58 > C > 95-104

This study aims to determine physio-chemical properties of tempoyak, characterise the various indigenous species of lactic acid bacteria (LAB) present at different stages of fermentation and also to determine the survival of selected foodborne pathogens in tempoyak. The predominant microorganisms present in tempoyak were LAB (8.88–10.42 log CFU/g). Fructobacillus durionis and Lactobacillus plantarum...

article

Effect of reduced graphene oxide-hybridized ZnO thin films on the photoinactivation of Staphylococcus aureus and Salmonella enterica serovar Typhi

Swe Jyan Teh, Soo Ling Yeoh, Kian Mun Lee, Chin Wei Lai, more

Journal of Photochemistry and Photobiology B: Biology > 2016 > 161 > C > 25-33

The immobilization of photocatalyst nanoparticles on a solid substrate is an important aspect for improved post-treatment separation and photocatalyst reactor design. In this study, we report the simple preparation of reduced graphene oxide (rGO)-hybridized zinc oxide (ZnO) thin films using a one-step electrochemical deposition, and investigated the effect of rGO-hybridization on the photoinactivation...

article

Epidemiology of human leptospirosis in Malaysia, 2004–2012

Douadi Benacer, Kwai Lin Thong, Ng Choung Min, Khebir Bin Verasahib, more

Acta Tropica > 2016 > 157 > C > 162-168

Leptospirosis is an emerging disease, especially in countries with a tropical climate such as Malaysia. A dramatic increase in the number of cases has been reported over the last decade; however, information on the epidemiological trends of this disease is lacking. The objective of this study is to provide an epidemiological description of human leptospirosis cases over a 9-year period (2004–2012)...

article

A microfluidic lab-on-a-disc integrated loop mediated isothermal amplification for foodborne pathogen detection

Abkar Ahmed Sayad, Fatimah Ibrahim, Shah Mukim Uddin, Koh Xiu Pei, more

Sensors and Actuators B: Chemical > 2016 > 227 > C > 600-609

Salmonellosis is one of the most common foodborne diseases caused by the genus Salmonella and is considered to be a significant global health concern. Therefore, the development of effective techniques for rapidly detecting Salmonella is fundamental in preventing foodborne outbreaks. This paper presents a lab-on-a-disc integrated loop mediated isothermal amplification (LAMP) on a microfluidic platform...

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INFONA - science communication portal

Search results for: Kwai-Lin Thong

Molecular characterization of Vibrio cholerae O1 El Tor strains in Malaysia revealed genetically diverse variant lineages

Pre-enrichment step, incubation temperature and type of selective media affect the pathogenic Vibrio parahaemolyticus detection efficiency in frozen prawns

Role of coaggregation in the pathogenicity and prolonged colonisation of Vibrio cholerae

Catabolic activity and biofilm formation of foodborne Listeria monocytogenes strains

Treatment of Cotton by β-Cyclodextrin/Triclosan Inclusion Complex and Factors Affecting Antimicrobial Properties

A microdevice for rapid, monoplex and colorimetric detection of foodborne pathogens using a centrifugal microfluidic platform

Development of an aptasensor using reduced graphene oxide chitosan complex to detect Salmonella

Genetic diversity, virulotyping and antimicrobial resistance susceptibility of Yersinia enterocolitica isolated from pigs and porcine products in Malaysia

Graphene-based label-free electrochemical aptasensor for rapid and sensitive detection of foodborne pathogen

Diversity of microbiota associated with symptomatic and non-symptomatic bacterial wilt-diseased banana plants determined using 16S rRNA metagenome sequencing

Salmonella Typhi genomics: envisaging the future of typhoid eradication

Prevalence of methicillin-resistant Staphylococcus aureus (MRSA) infection and the molecular characteristics of MRSA bacteraemia over a two-year period in a tertiary teaching hospital in Malaysia

Comparative genome analysis of multiple vancomycin-resistant Enterococcus faecium isolated from two fatal cases

Comparative genomics analyses revealed two virulent Listeria monocytogenes strains isolated from ready-to-eat food

Evaluation of basic knowledge on food safety and food handling practices amongst migrant food handlers in Peninsular Malaysia

Antagonistic effects of biofilm-forming bacterial strains co-inoculated with blood disease pathogenic strain, Ralstonia syzygii subspecies celebesensis in banana plants

Physio-chemical, microbiological properties of tempoyak and molecular characterisation of lactic acid bacteria isolated from tempoyak

Effect of reduced graphene oxide-hybridized ZnO thin films on the photoinactivation of Staphylococcus aureus and Salmonella enterica serovar Typhi

Epidemiology of human leptospirosis in Malaysia, 2004–2012

A microfluidic lab-on-a-disc integrated loop mediated isothermal amplification for foodborne pathogen detection

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