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Since SHG is generated by specific macromolecules, it can potentially detect early disruptions of stromal architecture. Recent pilot data suggest that neoplastic changes in feline skin can be identified based on their SHG optical signature.
The noncentrosymmetric molecular structures responsible for the second order nonlinear effects of collagen fibrils are identified by sum frequency vibrational spectroscopy. CH2 and C=O functional groups dominate the achiral and chiral spectra, respectively.
Transitions in order/disorder phase states of biological tissues were analyzed using polarization modulated second harmonic generation imaging. The degree of structural disorder was significantly correlated with severity of pathological changes.
An experimental method for quantifying disorder within the anulus fibrosus is described based on polarization-modulated second harmonic generation imaging (PM-SHG-I). This method is demonstrated by imaging the anular lamellar architecture of a mouse model of compressive loading. Results were consistent with those obtained in an earlier study where organization was quantified directed secants image...
Abstract. A preliminary single-animal study of in vivo argon laser vascular welding was conducted using a canine model. The effects of temperature feedback control and saline drip cooling on patency and collagen cross-linking were investigated. The surface temperature at the centre of the laser spot was monitored using a two-colour infrared thermometer. The surface temperature was limited by either...
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