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Aims: To develop and evaluate a TaqMan‐based internal amplification control (IAC) that can be used as DNA in real‐time PCR (qPCR) or as RNA in reverse transcription real‐time PCR (qRT‐PCR) to identify the presence of assay inhibition and to evaluate its incorporation into existing qPCR and qRT‐PCR methods for bacterial detection.
Methods and Results: A DNA IAC was constructed by generating a 198‐bp...
Pathogenic Vibrio parahaemolyticus (Vp) (tdh+/trh+) represent a small percentage of environmental Vp populations, and very little is known about this subpopulation. Repetitive extragenic palindromic PCR and multilocus sequence analysis revealed heterogeneity among 41 Vp containing thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) that were isolated from Mississippi coastal environments...
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