Search results for: Jianghua Li

Items from 1 to 20 out of 154 results

article

Combinatorial metabolic engineering of Bacillus subtilis for menaquinone‐7 biosynthesis

Xian Sun, Xinyu Bi, Guyue Li, Shixiu Cui, more

Biotechnology and Bioengineering > 121 > 10 > 3338 - 3350

Menaquinone‐7 (MK‐7), a form of vitamin K2, supports bone health and prevents arterial calcification. Microbial fermentation for MK‐7 production has attracted widespread attention because of its low cost and short production cycles. However, insufficient substrate supply, unbalanced precursor synthesis, and low catalytic efficiency of key enzymes severely limited the efficiency of MK‐7 synthesis....

article

Chemoenzymatic Synthesis of N,N,N‐Trimethyl‐D‐Glucosamine Chitotriomycin and Its Analogues

Jianghua Li, Yan Zhang, Zhifei Hu, Jinfeng Ye, more

Chinese Journal of Chemistry > 42 > 19 > 2293 - 2298

N,N,N‐Trimethyl‐D‐glucosamine (TMG)‐chitotriomycin, a naturally occurring chitin related oligosaccharide, is a specific β‐N‐acetylhexosaminidases (HexNAcases) inhibitor for insects and fungi. Although TMG‐chitoriomycin holds great promise as a novel class insecticide and fungicide, the limited accessibility of TMG‐chitotriomycin prevents its further biological evaluation. We report herein a simple...

article

Efficient biosynthesis of active hemoglobins through enhancing the import of heme in Saccharomyces cerevisiae

Fan Liu, Xiaoyan Sun, Jingwen Zhou, Jianghua Li, more

The FEBS Journal > 291 > 16 > 3737 - 3748

Hemoglobins, with heme as a cofactor, are functional proteins that have extensive applications in the fields of artificial oxygen carriers and foods. Although Saccharomyces cerevisiae is an ideal host for hemoglobin synthesis, it lacks a suitable transport system to utilize additional heme for active expression of hemoglobins, resulting in the cellular aggregation and degradation of the latter. Here,...

article

High‐activity recombinant human carboxypeptidase B expression in Pichia pastoris through rational protein engineering and enhancing secretion from the Golgi apparatus to the plasma membrane

Jia Li, Qinan Shao, Yulong Xiang, Jianghua Li, more

Biotechnology Journal > 19 > 5 > n/a - n/a

Human carboxypeptidase B1 (hCPB1) is vital for recombinant insulin production, holding substantial value in the pharmaceutical industry. Current challenges include limited hCPB1 enzyme activity. In this study, recombinant hCPB1 efficient expression in Pichia pastoris was achieved. To enhance hCPB1 secretion, we conducted signal peptides screening and deleted the Vps10 sortilin domain, reducing vacuolar...

article

Magnetic Second‐Order Topological Insulator: An Experimentally Feasible 2D CrSiTe₃

Xiaotian Wang, Xiao‐Ping Li, Jianghua Li, Chengwu Xie, more

Advanced Functional Materials > 33 > 49 > n/a - n/a

2D second‐order topological insulators (SOTIs) have sparked significant interest, but currently, the proposed realistic 2D materials for SOTIs are limited to nonmagnetic systems. In this study, for the first time, a single layer of chalcogenide CrSiTe₃—an experimentally realized transition metal trichalcogenide is proposed with a layer structure—as a 2D ferromagnetic (FM) SOTI. Based on first‐principles...

article

A‐DA’D‐A Type Pentacyclic Small Molecule Acceptors to Exceed 16.5% Efficiency by Heteroatom Effect at the Outer Side Chain

Shaofeng Zhu, Changzhou Shi, Qingya Wei, Can Zhu, more

Chinese Journal of Chemistry > 41 > 15 > 1815 - 1822

In this work, we adopt a “heteroatom side‐chains” modification strategy to modify the thiophene units in A‐DA'D‐A (acceptor‐donor‐acceptor’‐donor‐acceptor) type pentacyclic SMAs (small molecule acceptors), that is, introducing branched alkyl chain at the β‐position of thiophene instead of straight alkyl chain, and then introducing oxygen atom at the third‐position on the basis of branched chain. Two...

article

etiBsu1209: A comprehensive multiscale metabolic model for Bacillus subtilis

Xinyu Bi, Yang Cheng, Xianhao Xu, Xueqin Lv, more

Biotechnology and Bioengineering > 120 > 6 > 1623 - 1639

Genome‐scale metabolic models (GEMs) have been widely used to guide the computational design of microbial cell factories, and to date, seven GEMs have been reported for Bacillus subtilis, a model gram‐positive microorganism widely used in bioproduction of functional nutraceuticals and food ingredients. However, none of them are widely used because they often lead to erroneous predictions due to their...

article

Enzymatic Synthesis of Sialyl Lactosamine Grafted Chitooligosaccharides

Jun Yan, Jianghua Li, Zhifei Hu, Xiaoyao Ma, more

Chinese Journal of Chemistry > 41 > 11 > 1299 - 1304

An efficient enzymatic assembly strategy was developed for the concise synthesis of structurally well‐defined sialylated chitooligosaccharides. Two enzyme modules for the β‐1,3‐N‐acetyl‐glucosaminylation and β‐1,4‐galactosylation were applied for the grafting N‐acetyl lactosamine (LacNAc) unit(s) onto the chitooligosaccharides. The LacNAc grafted chitooligosaccharides were further modified with α‐2,3‐...

article

Cascade Catalytically Released Nitric Oxide‐Driven Nanomotor with Enhanced Penetration for Antibiofilm

Jia Zheng, Wei Wang, Xinyu Gao, Senfeng Zhao, more

Small > 18 > 52 > n/a - n/a

Nanodrugs are becoming increasingly important in the treatment of bacterial infection, but their low penetration ability to bacterial biofilm is still the main challenge hindering their therapeutic effect. Herein, nitric oxide (NO)‐driven nanomotor based on L‐arginine (L‐Arg) and gold nanoparticles (AuNPs) loaded dendritic mesoporous silica nanoparticles (AG‐DMSNs) is fabricated. AG‐DMSNs have the...

article

Model‐driven design of synthetic N‐terminal coding sequences for regulating gene expression in yeast and bacteria

Chenyun Wang, Wei Zhang, Rongzhen Tian, Jianing Zhang, more

Biotechnology Journal > 17 > 5 > n/a - n/a

N‐terminal coding sequences (NCSs) are key regulatory elements for fine‐tuning gene expression during translation initiation—the rate‐limiting step of translation. However, owing to the complex combinatory effects of NCS biophysical factors and endogenous regulation, designing NCSs remains challenging. In this study, a multi‐view learning strategy for model‐driven generation of synthetic NCSs for...

article

Engineered yeast for efficient de novo synthesis of 7‐dehydrocholesterol

Lisha Qu, Xiang Xiu, Guoyun Sun, Chenyang Zhang, more

Biotechnology and Bioengineering > 119 > 5 > 1278 - 1289

The synthesis of vitamin D3 precursor 7‐dehydrocholesterol (7‐DHC) by microbial fermentation has much attracted attention owing to its advantages of environmental protection. In this study, Saccharomyces cerevisiae was engineered for a de novo biosynthesis of 7‐DHC. First, seven essential genes (six endogenous genes and one heterologous gene) were overexpressed, and the ROX1 gene (heme‐dependent repressor...

article

The mechanism of multiple organ dysfunction syndrome in patients with COVID‐19

Wenbin Zhao, Hanmeng Li, Jianghua Li, Bin Xu, more

Journal of Medical Virology > 94 > 5 > 1886 - 1892

In late 2019, an outbreak of coronavirus disease 2019 (COVID‐19) arose, caused by severe acute respiratory syndrome coronavirus type 2 (SARS‐CoV‐2). This disease rapidly became a public health event of international concern. In addition to the most typical symptoms of dyspnea, numerous patients with COVID‐19 exhibited systemic symptoms, such as cardiovascular disease, liver and kidney failure, and...

article

Electrocardiographic localization of peripherally inserted central catheter tip position in critically ill patients with advanced cancer: An application study

Yong‐Hong Chai, Su‐Ya Han, Yu‐Xin Zhu, Jin‐Jie Hou, more

Annals of Noninvasive Electrocardiology > 27 > 2 > n/a - n/a

Background We compared the methods of electrocardiogram (ECG) and X‐ray localization of the peripherally inserted central catheter (PICC) tip position, in order to find a more convenient, practical, and safe method. Objective To investigate the value of applying electrocardiographic localization of the PICC tip position in critically ill patients with advanced cancer in Hebei Province, China. ...

article

Metaproteomic analysis of enzymatic composition in Baobaoqu fermentation starter for Wuliangye baijiu

Weiye Fan, Xinrui Zhao, Guocheng Du, Jian Chen, more

International Journal of Food Science & Technology > 56 > 8 > 4170 - 4181

Wuliangye is one of the most famous brands of baijiu in China. The typical flavours of Wuliangye baijiu are mainly formed by the multifarious enzymes in fermentation starter (Baobaoqu, BBQ). As the enzymes remained poorly understood, the detailed enzymatic composition of BBQ was analysed by metaproteomics for the first time. It is worth noting that the enzymes from plant, animals and micro‐organisms...

article

Conferring thermotolerant phenotype to wild‐type Yarrowia lipolytica improves cell growth and erythritol production

Xueliang Qiu, Yang Gu, Guocheng Du, Juan Zhang, more

Biotechnology and Bioengineering > 118 > 8 > 3117 - 3127

In microbial engineering, heat stress is an important environmental factor modulating cell growth, metabolic flux distribution and the synthesis of target products. Yarrowia lipolytica, as a GARS (generally recognized as safe) nonconventional yeast, has been widely used in the food industry, especially as the host of erythritol production. Biomanufacturing economics is limited by the high operational...

article

Engineering a thermostable chondroitinase for production of specifically distributed low‐molecular‐weight chondroitin sulfate

Hao Wang, Lin Zhang, Yang Wang, Jianghua Li, more

Biotechnology Journal > 16 > 5 > n/a - n/a

Chondroitinase ABC I (csABC I) has attracted intensive attention because of its great potential in heparin refining and the enzymatic preparation of low‐molecular‐weight chondroitin sulfate (LMW‐CS). However, low thermal resistance (<30℃) restricts its applications. Herein, structure‐guided and sequence‐assisted combinatorial engineering approaches were applied to improve the thermal resistance...

article

The elucidation of phosphosugar stress response in Bacillus subtilis guides strain engineering for high N‐acetylglucosamine production

Tengfei Niu, Xueqin Lv, Yanfeng Liu, Jianghua Li, more

Biotechnology and Bioengineering > 118 > 1 > 383 - 396

Bacillus subtilis is a preferred microbial host for the industrial production of nutraceuticals and a promising candidate for the synthesis of functional sugars, such as N‐acetylglucosamine (GlcNAc). Previously, a GlcNAc‐overproducer B. subtilis SFMI was constructed using glmS ribozyme dual‐regulatory tool. Herein, we further engineered to enhance carbon flux from glucose towards GlcNAc synthesis...

article

Biotransformation and chiral resolution of d,l‐alanine into pyruvate and d‐alanine with a whole‐cell biocatalyst expressing l‐amino acid deaminase

Ke Liu, Mengyue Gong, Xueqin Lv, Jianghua Li, more

Biotechnology and Applied Biochemistry > 67 > 4 > 668 - 676

Pyruvate is an important pharmaceutical intermediate and is widely used in food, nutraceuticals, and pharmaceuticals. However, high environmental pollution caused by chemical synthesis or complex separation process of microbial fermentation methods constrain the supply of pyruvate. Here, one‐step pyruvate and d‐alanine production from d,l‐alanine by whole‐cell biocatalysis was investigated. First,...

article

Development and optimization of N‐acetylneuraminic acid biosensors in Bacillus subtilis

Xiaolong Zhang, Yanting Cao, Yanfeng Liu, Long Liu, more

Biotechnology and Applied Biochemistry > 67 > 4 > 693 - 705

Transcriptional factor (TF)‐based metabolite‐responsive biosensors are important tools for screening engineered enzymes with desired properties and for the dynamic regulation of biosynthetic pathways. However, TF‐based biosensor construction is often constrained by undesired effects of TF‐binding site sequence insertion on gene expression and unpredictable optimal TF expression levels. In the present...

article

Systems metabolic engineering of Bacillus subtilis for efficient biosynthesis of 5‐methyltetrahydrofolate

Han Yang, Yanfeng Liu, Jianghua Li, Long Liu, more

Biotechnology and Bioengineering > 117 > 7 > 2116 - 2130

5‐Methyltetrahydrofolate (5‐MTHF) is the major form of folate in human plasma and is the only folate form that can penetrate the blood–brain barrier. It has been widely used for the prevention and treatment of various diseases. It is mainly produced by chemical synthesis. However, the low production rate cannot meet the increasing demand. In addition, chemical synthesis is potentially detrimental...

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INFONA - science communication portal

Search results for: Jianghua Li

Combinatorial metabolic engineering of Bacillus subtilis for menaquinone‐7 biosynthesis

Chemoenzymatic Synthesis of N,N,N‐Trimethyl‐D‐Glucosamine Chitotriomycin and Its Analogues

Efficient biosynthesis of active hemoglobins through enhancing the import of heme in Saccharomyces cerevisiae

High‐activity recombinant human carboxypeptidase B expression in Pichia pastoris through rational protein engineering and enhancing secretion from the Golgi apparatus to the plasma membrane

Magnetic Second‐Order Topological Insulator: An Experimentally Feasible 2D CrSiTe₃

A‐DA’D‐A Type Pentacyclic Small Molecule Acceptors to Exceed 16.5% Efficiency by Heteroatom Effect at the Outer Side Chain

etiBsu1209: A comprehensive multiscale metabolic model for Bacillus subtilis

Enzymatic Synthesis of Sialyl Lactosamine Grafted Chitooligosaccharides

Cascade Catalytically Released Nitric Oxide‐Driven Nanomotor with Enhanced Penetration for Antibiofilm

Model‐driven design of synthetic N‐terminal coding sequences for regulating gene expression in yeast and bacteria

Engineered yeast for efficient de novo synthesis of 7‐dehydrocholesterol

The mechanism of multiple organ dysfunction syndrome in patients with COVID‐19

Electrocardiographic localization of peripherally inserted central catheter tip position in critically ill patients with advanced cancer: An application study

Metaproteomic analysis of enzymatic composition in Baobaoqu fermentation starter for Wuliangye baijiu

Conferring thermotolerant phenotype to wild‐type Yarrowia lipolytica improves cell growth and erythritol production

Engineering a thermostable chondroitinase for production of specifically distributed low‐molecular‐weight chondroitin sulfate

The elucidation of phosphosugar stress response in Bacillus subtilis guides strain engineering for high N‐acetylglucosamine production

Biotransformation and chiral resolution of d,l‐alanine into pyruvate and d‐alanine with a whole‐cell biocatalyst expressing l‐amino acid deaminase

Development and optimization of N‐acetylneuraminic acid biosensors in Bacillus subtilis

Systems metabolic engineering of Bacillus subtilis for efficient biosynthesis of 5‐methyltetrahydrofolate

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