Search results for: Gopal Pande

Items from 1 to 19 out of 19 results

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Strontium content and collagen‐I coating of Magnesium–Zirconia–Strontium implants influence osteogenesis and bone resorption

Dolly Mushahary, Cuie Wen, Jerald M. Kumar, Ragamouni Sravanthi, more

Clinical Oral Implants Research > 27 > 2 > e15 - e24

Objective Our objective was to study the role of Collagen type‐I (Col‐I) coating on Magnesium–Zirconia (Mg–Zr) alloys, containing different quantities of Strontium (Sr), in enhancing the in vitro bioactivity and in vivo bone‐forming and mineralisation properties of the implants. Materials and methods MC3T3‐E1 osteoblast cell line was used to analyse the in vitro properties of Col‐I coated and uncoated...

article

Collagen type-I leads to in vivo matrix mineralization and secondary stabilization of Mg–Zr–Ca alloy implants

Dolly Mushahary, Cuie Wen, Jerald Mahesh Kumar, Jixing Lin, more

Colloids and Surfaces B: Biointerfaces > 2014 > 122 > C > 719-728

Biodegradable magnesium–zirconia–calcium (Mg–Zr–Ca) alloy implants were coated with Collagen type-I (Coll-I) and assessed for their rate and efficacy of bone mineralization and implant stabilization. The phases, microstructure and mechanical properties of these alloys were analyzed using X-ray diffraction (XRD), optical microscopy and compression test, respectively, and the corrosion behavior was...

article

Synthesis and functional characterization of a fluorescent peptide probe for non invasive imaging of collagen in live tissues

Ushasri Chilakamarthi, Jaipal Kandhadi, Srinivas Gunda, Avinash Raj Thatipalli, more

Experimental Cell Research > 2014 > 327 > 1 > 91-101

Targeted molecular imaging to detect changes in the structural and functional organization of tissues, at the molecular level, is a promising approach for effective and early diagnosis of diseases. Quantitative and qualitative changes in type I collagen, which is a major component in the extra cellular matrix (ECM) of skin and other vital organs like lung, liver, heart and kidneys, are often associated...

article

Strategies to prepare TiO2 thin films, doped with transition metal ions, that exhibit specific physicochemical properties to support osteoblast cell adhesion and proliferation

Marshal Dhayal, Renu Kapoor, Pavana Goury Sistla, Ravi Ranjan Pandey, more

Materials Science & Engineering C > 2014 > 37 > Complete > 99-107

Metal ion doped titanium oxide (TiO ₂ ) thin films, as bioactive coatings on metal or other implantable materials, can be used as surfaces for studying the cell biological properties of osteogenic and other cell types. Bulk crystallite phase distribution and surface carbon–oxygen constitution of thin films, play an important role in determining the biological responses of cells that come in...

article

Multifunctional gradient coatings of phosphate-free bioactive glass on SS316L biomedical implant materials for improved fixation

Jui Chakraborty, Somoshree Sengupta, Sayantan Ray, Swapankumar Ghosh, more

Surface & Coatings Technology > 2014 > 240 > Complete > 437-443

A simple technique of applying porous (2–15μm pore diameter) bi-layered vitreous coatings with phosphate free bioactive glass (PFBG) on surgical grade SS316L substrates by conventional spray coating method is demonstrated. The adhesion strength of the topmost porous layer (55μm thick) of PFBG to the underlying SS316L substrate was improved by providing an impervious 10–15μm thick PFBG glass interface...

article

Cell biological responses of osteoblasts on anodized nanotubular surface of a titanium‐zirconium alloy

Subhash Sista, Alireza Nouri, Yuncang Li, Cuie Wen, more

Journal of Biomedical Materials Research Part B: Applied Biomaterials > 101A > 12 > 3416 - 3430

Anodization of titanium and its alloys, under controlled conditions, generates a nanotubular architecture on the material surface. The biological consequences of such changes are poorly understood, and therefore, we have analyzed the cellular and molecular responses of osteoblasts that were plated on nanotubular anodized surface of a titanium‐zirconium (TiZr) alloy. Upon comparing these results with...

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Histological analysis of cells and matrix mineralization of new bone tissue induced in rabbit femur bones by Mg–Zr based biodegradable implants

Sravanthi Ragamouni, Jerald Mahesh Kumar, Dolly Mushahary, Harishankar Nemani, more

Acta Histochemica > 2013 > 115 > 7 > 748-756

The biological efficacy of bone inducing implant materials in situ can be assessed effectively by performing histological analysis. We studied the peri-implant bone regeneration around two types of biodegradable magnesium–zirconium alloys, Mg–5Zr and Mg–Zr–2Sr, using histological, histochemical and immunohistochemical methods in the femur of New Zealand White strain rabbits. Our study includes three...

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Expression of cell adhesion and differentiation related genes in MC3T3 osteoblasts plated on titanium alloys: role of surface properties

Subhash Sista, Cuie Wen, Peter D. Hodgson, Gopal Pande

Materials Science & Engineering C > 2013 > 33 > 3 > 1573-1582

It is important to understand the cellular and molecular events that take place at the cell–material interface of implants used for bone repair. An understanding of the mechanisms involved in the initial stages of osteoblast interactions with the surface of the implant material is fundamental in deciding the fate of the cells that come in contact with it. In this study, we compared the relative gene...

article

Mg–Zr–Sr alloys as biodegradable implant materials

Yuncang Li, Cuie Wen, Dolly Mushahary, Ragamouni Sravanthi, more

Acta Biomaterialia > 2012 > 8 > 8 > 3177-3188

Novel Mg–Zr–Sr alloys have recently been developed for use as biodegradable implant materials. The Mg–Zr–Sr alloys were prepared by diluting Mg–Zr and Mg–Sr master alloys with pure Mg. The impact of Zr and Sr on the mechanical and biological properties has been thoroughly examined. The microstructures and mechanical properties of the alloys were characterized using optical microscopy, X-ray diffraction...

article

Growth, differentiation, and migration of osteoblasts on transparent Ni doped TiO₂ thin films deposited on borosilicate glass

Marshal Dhayal, Renu Kapoor, Pavana Goury Sistla, Chander Kant, more

Journal of Biomedical Materials Research Part B: Applied Biomaterials > 100A > 5 > 1168 - 1178

A simple and cost effective dip coating method was used to deposit thin films of amorphous (AM) or anatase (AN) titanium dioxide (TiO₂) on borosilicate glass substrates, either with or without prior doping of TiO₂ with nickel (Ni) cations by a specially designed sol gel technique. The objective of the study was to compare the physicochemical and biological properties of these films and assess their...

article

Lipopeptide with a RGDK Tetrapeptide Sequence Can Selectively Target Genes to Proangiogenic α5β1 Integrin Receptor and Mouse Tumor Vasculature

Dipankar Pramanik, Bharat K. Majeti, Goutam Mondal, Priya P. Karmali, more

Journal of Medicinal Chemistry > 2008 > 51 > 22 > 7298 - 7302

article

Direct association between caspase 3 and α5β1 integrin and its role during anoikis of rat fibroblasts

J. Rajeswari, Gopal Pande

Cell Biology International > 2006 > 30 > 12 > 963-969

We have demonstrated a direct association between α5β1 integrin and caspase 3, both pro- and mature enzyme, in various sub-cellular compartments of rat fibroblasts undergoing anoikis. Integrin associated caspase 3 showed high activity in the plasma membranes, whereas in the cytosol and microsomal fraction it showed little or no activity. Our results suggest a possible role for recycled un-ligated...

article

Cholesterol alters the interaction of glycosphingolipid GM3 with α5β1 integrin and increases integrin-mediated cell adhesion to fibronectin

Praturi Gopalakrishna, Nandini Rangaraj, Gopal Pande

Experimental Cell Research > 2004 > 300 > 1 > 43-53

Integrins bind to their ligand in the extracellular matrix (ECM), such as fibronectin (FN), through a specific interaction between the amino acid motifs in the ligand, and binding sites in the extracellular domains of the integrin molecule generated jointly by its α and β subunits. It has been proposed that membrane cholesterol and glycosphingolipids (GSLs) can regulate integrin–ECM interactions and...

article

A correlation between membrane cholesterol level, cell adhesion and tumourigenicity of polyoma virus transformed cells

Tanvir Kaur, Praturi Gopalakrishna, Pulicat Subramanian Manogaran, Gopal Pande

Molecular and Cellular Biochemistry > 2004 > 265 > 1-2 > 83-95

We have investigated the implications of the rise in membrane cholesterol levels on several in vitro and in vivo properties of polyoma virus transformed rat fibroblasts (PyF), with a special emphasis on α₅β_{_1} integrin functions. We show that increased membrane cholesterol causes the PyF cells to change their shape and become more bipolar in appearance. These cells also show significantly higher adhesion...

article

THE SIGNIFICANCE OF α5β1 INTEGRIN DEPENDENT AND INDEPENDENT ACTIN CYTOSKELTON ORGANIZATION IN CELL TRANSFORMATION AND SURVIVAL

J. Rajeswari, Gopal Pande

Cell Biology International > 2002 > 26 > 12 > 1043-1055

Cell-matrix and cell-cell interactions are important physiological determinants of cell growth, survival and transformation. Cell adhesion to the extra cellular matrix (ECM) via integrins also crucially influences the organization of the cytoskeleton. It triggers a cascade of intracellular biochemical events, which regulate cell viability and growth. We have studied the relationship between cell attachment...

article

Correlates of developmental cell death in Dictyostelium discoideum

Trupti Kawli, B.R. Venkatesh, Vidyanand Nanjundiah, P. Kevin Kennady, more

Differentiation > 2002 > 70 > 6 > 272-281

We have studied the correlates of cell death during stalk cell differentiation in Dictyostelium discoideum. Our main findings are four. (i) There is a gradual increase in the number of cells with exposed phosphatidyl serine residues, an indicator of membrane asymmetry loss and increased permeability. Only presumptive stalk cells show this change in membrane asymmetry. Cells also show an increase in...

article

The role of membrane lipids in regulation of integrin functions

Gopal Pande

Current Opinion in Cell Biology > 2000 > 12 > 5 > 569-574

Recent evidence suggests that the biochemical and physical organization of lipid molecules in the plasma membrane can affect integrin-mediated cellular functions. The nature and mechanism of integrin-lipid interactions are unknown, but it is clear that they play specific roles in modulating the properties of integrins and integrin-associated proteins. A better knowledge of integrin functions, especially...

article

Heterogeneity of DNA content and expression of cell cycle genes in axenically growing Entamoeba histolytica HM1:IMSS clone A

Samudra S. Gangopadhyay, Sib Sankar Ray, Kavin Kennady, Gopal Pande, more

Molecular & Biochemical Parasitology > 1997 > 90 > 1 > 9-20

The cell division cycle of Entamoeba histolytica was studied using multi-parametric flow cytometry in asynchronous and partially synchronised cells. Dynamic changes in the DNA synthesis and DNA content of axenically growing trophozoites were observed by using 5-bromo-2′-deoxyuridine (BrdU) uptake and DNA specific fluorochromes. It was observed that DNA synthesis in these cells continues beyond the...

article

Primary structure of Entamoeba histolytica γ-tubulin and localisation of amoebic microtubule organising centres

Sib Sankar Ray, Samudra S Gangopadhyay, Gopal Pande, John Samuelson, more

Molecular & Biochemical Parasitology > 1997 > 90 > 1 > 331-336

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