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Anemia, a condition characterized by insufficient hemoglobin, affects 56.2% of pregnant women and 66.1% of children under five in low-resource countries. Though hemoglobin concentration measurement is the most common laboratory test in the world, the high cost of disposables (>$1.00 per test in Malawi) limits its availability in these settings. We have demonstrated a spectrophotometric method that...
In this study, a qualitative human immunodeficiency virus-1 (HIV-1) diagnostic assay was developed and evaluated as part of an effort to create a point-of-care diagnostic test for pediatric HIV-1. The assay is based on extraction of viral ribonucleic acid (RNA) with magnetic microbeads, isothermal amplification using nucleic acid sequence based amplification (NASBA), and detection of amplified RNA...
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