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We propose a microrobotic platform that stimulates swimming microorganisms in a microfluidic chip with high speed and accuracy. The developed platform comprises (1) high-speed microrobots that can generate a millinewton-level driving force, micrometer-level positioning accuracy, and a millimeter per second level drive speed through the actuation of motorized stages with permanent magnets and (2) high-speed...
Bio-actuated, especially flagellated bacteria-driven, micro/nanorobots have been actively studied to develop novel technologies such as drug delivery systems (DDS), though the characteristics of the actuator (flagellar motor) have not been sufficiently investigated. For analysis or control of the bacterial flagellar motor, we previously proposed the local environmental control system with nano/micro...
In this paper, we discuss a novel, magnetically driven microrobot equipped with a frame structure to measure the stimulant property of aquatic microorganisms. The fabricated microfluidic chip and microrobots are shown. The untethered microrobot is composed of a Si-Ni hybrid structure constructed by MEMS techniques. The microrobots with 5 μm width force sensors are actuated in a microfluidic chip by...
Bio-actuated, especially flagellated bacteria-driven, microrobots have been actively studied to develop novel technologies such as microsurgery inside human body, though the characteristics of the bacterial flagellar motor have not been sufficiently investigated. For analysis of the flagellar motor, we previously proposed the local environmental control system with nano/micro dual pipettes to change...
In this paper, we introduce an untethered type of magnetically driven microtool (MMT) with a force sensing function to investigate the stimulus characteristics of aquatic microorganisms in a microfluidic chip. The microchip is composed of the proposed MMTs, which have a Si-Ni hybrid structure, a transparent cover made of polydimethylsiloxane (PDMS), a glass substrate, and a microspacer to prevent...
Micro/nano robots have been actively studied to lead the developments of the novel technologies, such as drug delivery systems (DDS) and micro/nano surgery inside human body. Recently, the micro living organisms, especially flagellated bacteria, have been used as the driving forces for the microobjects. To achieve the more precise control of the bacteria-driven microobjects, we previously developed...
In this paper, we propose 3DOF dual-arm microrobots which enabling manipulation and force sensing for a single cell of microorganisms in a microfluidic chip. The untethered microrobots are actuated by permanent magnets which can supply mN order force to stimulate microorganisms and measure the applied force where we want to measure them. The design and fabrication of the untethered microrobots with...
Micro/Nano robots have attracted scientific attention to develop novel technologies such as drug delivery systems. Recently, flagellated bacteria have been used as the driving force of microobjects to develop bacteria-driven microrobots. In this paper, we propose a method of smart manipulation of multiple bacteria-driven microobjects with single manipulated guiderobot based on bacterial autonomous...
Micro/nano robots have been actively studied to lead the developments of the novel technologies, such as drug delivery systems (DDS) and micro/nano surgery inside human body. Recently, the micro living organisms, especially flagellated bacteria, have been used as the driving forces for the microobjects. To achieve the more precise control of the bacteria-driven microobjects, we previously developed...
We propose in situ formation of gel microbeads made of a thermoreversible hydrogel for indirect laser micromanipulation of microorganisms, DNA and viruses. Irradiation, using a 1064 nm laser, of an aqueous solution mixed with poly-(N-isopropylacrylamide) through a high magnification lens resulted in the formation of a gel microbead at the laser focus due to heating. The gel microbead was trapped by...
Isolation and separation of the target microbial cells is quite important in applied microbiology. We propose a new touch sensor for separation of the target cells on the plate using a micromanipulator with a glass pipette. Principle of this touch sensor is based on detection of mechanical impedance change after contact of the vibrating pipette tip with the plate. We fixed the sensor on the pipette...
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