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A glycolate oxidase (GO) gene was obtained by reverse transcription-polymerase chain reaction (RT-PCR) from the spinach leaves, and the recombinant E. coli BL21(DE3)/pET-GO was constructed. To know whether bacterial hemoglobin can be used as an oxygen donor to oxygen-dependent GO, Vitreoscilla hemoglobin (VHb) gene was fused with GO gene by overlap-PCR and the fusion protein VGO was expressed in E...
Based on the performance requirements of dust suppressant for the open-pit mine road, this paper determined that moisture-absorption dust suppressant contains hygroscopic agent, coagulation agent and accessory ingredient. The optimal formula was developed through orthogonal experiment. Moisture content in dust sample, sprayed with the dust suppressant was 13.4% after the dust sample was left untouched...
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