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Multifocal multiphoton laser scanning microscopy (mfMPLSM) in the biological and medical sciences has the potential to become a ubiquitous tool for obtaining high-resolution images at video rates. While current implementations of mfMPLSM achieve very high frame rates, they are limited in their applicability to essentially those biological samples that exhibit little or no scattering. In this paper,...
We apply remote focusing to multi-focal multiphoton microscopy by simultaneously imaging multiple focal planes of Drosophila melanogaster olfactory neurons. This technology permits imaging the entire volume of the antennal lobe in a single scan.
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