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To develop a genetically engineered Escherichia coli for potential mass production of antibacterial peptide salmine instead of extraction from fish sperm, three primers designed according to amino acid sequence of salmine were used to construct an oligonucleotide fragment (SAL) encoding salmine. SAL was inserted into a pEC vector and transformed into E. coli BL21 (DE3) to construct genetically engineered...
BACKGROUND: Fish skin, a by‐product of the food industry, contains a large amount of collagen. However, only a small proportion of fish skin is used in the production of leather materials and animal feedstuffs, most of it being discarded. The aims of this study were to prepare peptides from Alaska pollack (Theragra chalcogramma) skin by enzymatic hydrolysis and to evaluate the antioxidant activity...
Lactoferricin (Lfcin) is an amphipathic and cationic peptide with strong antibacterial activities against a broad range of Gram-positive, Gram-negative bacteria. It is usually generated by the pepsin-mediated digestion of lactoferrin. This paper investigated the potential for production of bovine lactoferricin (LfcinB) through genetic engineering. Three primers were designed according to amino acid...
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