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Abstracted by Tris-HCl buffer,pH8.5, treated by n-butanol, and fractioned by (NH4)2SO4 (60%) ,the alkaline phosphatase( AKP) from guts of Hypophthalmichthys molitrix Cuv.et Val was separated through DEAE-Sepharose and Sephacryl S-200. The purification folds was 150.08,while the specific activity was 4031.15 U/mg.The properties research suggested that the optimal pH and temperature were pH10.2 and...